Ethanol impairs migration of the prechordal plate in the zebrafish embryo
Ethanol impairs migration of the prechordal plate in the zebrafish embryo
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DOI：
10.1006/dbio.1998.8995
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发表时间：
1998-09-15
影响因子：
2.7
通讯作者：
Strähle, U
中科院分区：
文献类型：
--
作者：
Blader, P;Strähle, U
脊椎动物胚胎暴露于乙醇中会导致独眼，但对这种影响的潜在机制知之甚少。在这里，我们发现斑马鱼可以在早期原肠期通过短暂的乙醇处理诱导独眼，并伴有前脑和中脑腹侧基因表达特征的丧失。有趣的是，腹侧脑标记的表达缺陷与脊索前板中胚层的迁移受损有关，这表明斑马鱼胚胎中脊索前板中胚层在前神经板下的正确位置是确定前神经中线所必需的。然而，乙醇诱导的独眼一般不会损害前神经外胚层结构的诱导。最后,;尽管表达细胞的位置异位，但goosecoid和胰岛-1等基因在脊索前板细胞中表达的时间模式与对照胚胎相似，因此似乎脊索前板特异性基因表达的调控在很大程度上与脊索前板的最终位置无关。(C) 1998学术出版社。
Exposure of vertebrate embryos to ethanol causes cyclopia, but little is known about the underlying mechanisms of this effect. Here we show that cyclopia can be induced in the zebrafish by a short ethanol treatment during early gastrula stages and is accompanied by loss of gene expression characteristic of the ventral aspects of the fore- and midbrain. Interestingly, defects in the expression of ventral brain markers are linked to impaired migration of the prechordal plate mesoderm indicating that the correct position of the prechordal plate mesoderm under the anterior neural plate in the zebrafish embryo is required for specification of the anterior neural midline. Ethanol-induced cyclopia does not, however, impair the induction of anterior neuroectodermal structures in general. Finally,;as genes like goosecoid and islet-1 are expressed in prechordal plate cells in a temporal pattern similar to control embryos despite the ectopic position of expressing cells, it appears that regulation of prechordal plate-specific gene expression is largely independent of the final position of the prechordal plate. (C) 1998 Academic Press.