A comparison of the character of algal extracellular versus cellular organic matter produced by cyanobacterium, diatom and green alga
A comparison of the character of algal extracellular versus cellular organic matter produced by cyanobacterium, diatom and green alga
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DOI：
10.1016/j.watres.2013.12.022
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发表时间：
2014-03-15
期刊：
影响因子：
12.8
通讯作者：
Kopecka, Ivana
中科院分区：
文献类型：
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作者：
Pivokonsky, Martin;Safarikova, Jana;Kopecka, Ivana
研究了以铜绿微囊藻、硅藻Fragilaria crotonsis和绿藻衣藻Chlamydonion geitleri为优势种的3种藻类有机质(AOM)的特征。通过细胞计数、光密度和溶解有机碳浓度的测量来监测藻类的生长。用比紫外线吸光度(Suva)、多肽/蛋白质和非肽含量、疏水性和相对分子质量(MW)表征了指数生长期和平稳生长期释放的胞外有机物(EOM)和细胞有机物(COM)。结果表明，EOM和COM均以亲水性为主，Suva较低。与EOM相比，COM的多肽/蛋白质含量更丰富，亲水性更强(亲水比例约为89%)，Suva含量更低。分子量分级结果表明，这三个物种的EOM和COM都含有大量低分子量(100 KDa)的多糖。多肽/蛋白质的相对分子质量分布比非多肽的窄，并且随着培养时间的增加而变宽。铜绿假单胞菌COM中的多肽/蛋白质含量最高，其中高相对分子质量的多肽/蛋白质含量最高(22%)。这些结果表明，对AOM组成和特性的了解预先决定了哪种工艺在处理AOM含水方面将是有效的。(C)2013爱思唯尔有限公司。保留所有权利。
This study investigated characteristics of algal organic matter (AOM) derived from three species (cyanobacterium Microcystis aeruginosa, diatom Fragilaria crotonensis and green alga Chlamydonionas geitleri) which dominate phytoplanktonic populations in reservoirs supplying drinking water treatment plants. Algal growth was monitored by cell counting, optical density and dissolved organic carbon concentration measurements. Extracellular organic matter (EOM) released at exponential and stationary growth phases and cellular organic matter (COM) were characterised in terms of specific UV absorbance (SUVA), peptide/protein and non-peptide content, hydrophobicity and molecular weight (MW). It was found that both EOM and COM were predominantly hydrophilic with low SUVA. COM was richer in peptides/proteins, more hydrophilic (with about 89% of hydrophilic fraction for all three species) and had lower SUVA than EOM. MW fractionation showed that both EOM and COM of all three species contain large portions of low-MW (100 kDa) polysaccharides. Peptides/proteins exhibited narrower MW distribution than non-peptide fraction and it widened as the cultures grew. The highest amount of peptides/proteins with a significant portion of high-MW ones (22%) was observed in COM of M. aeruginosa. The results imply that the knowledge of AOM composition and characteristics predetermine which processes would be effective in the treatment of AOM laden water. (C) 2013 Elsevier Ltd. All rights reserved.