Serial femtosecond crystallography approaches to understanding catalysis in iron enzymes.
Serial femtosecond crystallography approaches to understanding catalysis in iron enzymes.
复制标题
串行飞秒晶体学方法可了解铁酶的催化作用。
DOI：
10.1016/j.sbi.2022.102486
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发表时间：
2022
影响因子：
6.8
通讯作者：
Worrall JAR
中科院分区：
文献类型：
--
作者：
Worrall JAR
具有含铁活性部位的酶在催化大量对有氧生命至关重要的氧化反应中起着至关重要的作用。确定静止状态、氧结合中间状态和底物结合状态下的铁酶的三维结构特别具有挑战性，尤其是因为Fe(III)和Fe(IV)氧化还原状态对强X射线或电子束引起的辐射诱导化学非常敏感。X射线自由电子激光等新型光源的出现，使得能够有效地摆脱辐射诱导化学效应的结构，并使时间分辨结构得以确定。对这两种应用都很重要的是能够获得晶体光谱数据，以确定铁在任何特定结构或时间点的氧化还原状态。
Enzymes with iron-containing active sites play crucial roles in catalysing a myriad of oxidative reactions essential to aerobic life. Defining the three-dimensional structures of iron enzymes in resting, oxy-bound intermediate and substrate-bound states is particularly challenging, not least because of the extreme susceptibility of the Fe(III) and Fe(IV) redox states to radiation-induced chemistry caused by intense X-ray or electron beams. The availability of novel sources such as X-ray free electron lasers has enabled structures that are effectively free of the effects of radiation-induced chemistry and allows time-resolved structures to be determined. Important to both applications is the ability to obtainin crystallospectroscopic data to identify the redox state of the iron in any particular structure or timepoint.