Probing the metabolic heterogeneity of live Euglena gracilis with stimulated Raman scattering microscopy
Probing the metabolic heterogeneity of live Euglena gracilis with stimulated Raman scattering microscopy
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DOI：
10.1038/nmicrobiol.2016.124
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发表时间：
2016-10-01
影响因子：
28.3
通讯作者：
Ozeki, Yasuyuki
中科院分区：
文献类型：
--
作者：
Wakisaka, Yoshifumi;Suzuki, Yuta;Ozeki, Yasuyuki
了解活的微藻中的代谢对于有效的生物材料工程是至关重要的，但是由于标记要求和有限的成像速度，常规方法无法评估能动微藻的异质群体。在这里，我们展示了无标记的视频速率代谢物成像的活眼虫gracilis和细胞内代谢物分布在不同的培养条件下的统计分析。我们的方法为理解微藻异质性、优化培养方法和筛选突变微藻提供了进一步的见解。
Understanding metabolism in live microalgae is crucial for efficient biomaterial engineering, but conventional methods fail to evaluate heterogeneous populations of motile microalgae due to the labelling requirements and limited imaging speeds. Here, we demonstrate label-free video-rate metabolite imaging of live Euglena gracilis and statistical analysis of intracellular metabolite distributions under different culture conditions. Our approach provides further insights into understanding microalgal heterogeneity, optimizing culture methods and screening mutant microalgae.