Effect of lead sulfide nanoparticles exposure on calcium homeostasis in rat hippocampus neurons
Effect of lead sulfide nanoparticles exposure on calcium homeostasis in rat hippocampus neurons
复制标题
硫化铅纳米粒子暴露对大鼠海马神经元钙稳态的影响
DOI：
10.1016/j.jinorgbio.2013.05.008
复制
发表时间：
2013-09-01
影响因子：
3.9
通讯作者：
Cai, Yuan
中科院分区：
文献类型：
--
作者：
Cao, Yanhua;Liu, Huajie;Cai, Yuan
PbS纳米粒子(NPs)是一种重要的生物医学生物成像材料。然而，考虑到铅元素的高毒性，特别是神经毒性，关键是要充分研究“裸”PbS纳米粒子的毒性评估。在本研究中，我们通过测量体重和脑系数的变化，在Y-电迷宫中检测记忆行为，以及对海马神经元超微结构和病理学的研究，系统地探讨了PBS纳米颗粒对大鼠的神经毒性作用。此外，为了探讨其毒性机制，我们还测定了不同脏器中铅和钙的含量，并观察了钙离子-三磷酸腺苷酶活性和L钙通道亚单位的表达。我们的结果证实了PbS纳米颗粒具有较高的神经毒性，其可能的机制可能是由于PbS纳米颗粒引起的钙转运异常所致的钙稳态紊乱。(C)2013 Elsevier Inc.保留所有权利。
PbS nanoparticles (NPs) is an important nanomaterial for biomedical imaging in living tissues. However, concerning the high toxicity, especially neurotoxicity, of Pb element, it is crucial that the toxicity assessment of "naked" PbS NPs should be adequately studied. In the current study, we systematically explored the neurotoxicity of PbS NPs in rats by measuring the body weight and brain coefficient changes, testing memory behaviors in Y-electric maze, and studying the neuronal ultrastructure and pathology in hippocampus. Furthermore, in order to study the toxic mechanism, we performed Pb and Ca content measurements in various organs, and investigated Ca2+-ATPase activity and L-type calcium channel subunit expression. Our results confirmed that PbS NPs showed high neurotoxicity, while a possible mechanism was suggested to be due to the PbS NPs-induced calcium homeostasis disorder which was caused by the abnormal calcium transportation. (C) 2013 Elsevier Inc. All rights reserved.