Single embryoid body formation in a multi-well plate
Single embryoid body formation in a multi-well plate
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DOI：
10.2225/vol10-issue2-fulltext-2
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发表时间：
2007-04-01
影响因子：
2.7
通讯作者：
Heuertz, Rita M
中科院分区：
文献类型：
--
作者：
Ezekiel, Uthayashanker R;Muthuchamy, Mariappan;Heuertz, Rita M
由小鼠胚胎干细胞（ES）形成的拟胚体（EB）概括了发育中胚胎的许多方面。特别重要的是，EB的同步分化概括了器官特异性发育，并通过形成均匀大小的EB在培养中实现。本文所述的方法证明了一种从小鼠ES细胞产生EB的简单且具有成本效益的方法。单个EB以多孔板形式形成，并且使用96孔多孔板产生大量EB。在多孔中形成的均匀的单一尺寸的EB是用于筛选化合物和确定分化效果的理想系统。由于EB包含所有三个种系，因此它们适合研究小分子对ES分化的影响，例如在高通量筛选方案中进行。
Embryoid bodies (EB) formed from murine embryonic stem (ES) cells recapitulate many aspects of a developing embryo. Of specific importance, synchronous differentiation of EB recapitulates organ-specific development and is achieved in culture by formation of uniformly sized EB. The method described here demonstrates a simple and cost-effective way of generating EB from murine ES cells. Single EB are formed in a multi-well plate format and large numbers of EB are generated using a 96-well multi-well plate. Uniform single-sized EB formed in the multi-well are an ideal system for screening compounds and determining differentiation effects. Since EB contain all three germ layers, they are appropriate for studying small molecule effects on differentiation of ES such as is performed in high-throughput screening protocols.