IDENTIFICATION OF DOUBLE HOLLIDAY JUNCTIONS AS INTERMEDIATES IN MEIOTIC RECOMBINATION
IDENTIFICATION OF DOUBLE HOLLIDAY JUNCTIONS AS INTERMEDIATES IN MEIOTIC RECOMBINATION
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DOI：
10.1016/0092-8674(95)90191-4
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发表时间：
1995-12-01
期刊：
影响因子：
64.5
通讯作者：
KLECKNER, N
中科院分区：
文献类型：
--
作者：
SCHWACHA, A;KLECKNER, N
在减数分裂期间，含有来自双亲染色体的信息的分支DNA分子在体内发生在减数分裂特异性双链断裂发生的位点处。我们在这里证明，这些联合分子是重组中间体：它们包含单链，已经经历了信息交换。此外，这些联合分子被分解成父母和重组双链体时，在体外处理与霍利迪连接解析核酸内切酶RuvC或T4 endo VII。结合以前的观察，这些结果强烈表明，关节分子是双霍利迪结。
During meiosis, branched DNA molecules containing information from both parental chromosomes occur in vivo at loci where meiosis-specific double-stranded breaks occur. We demonstrate here that these joint molecules are recombination intermediates: they contain single strands that have undergone exchange of information. Moreover, these joint molecules are resolved into both parental and recombinant duplexes when treated in vitro with Holliday junction-resolving endonucleases RuvC or T4 endo VII. Taken together with previous observations, these results strongly suggest that joint molecules are double Holliday junctions.