COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT
COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT
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DOI：
10.1126/science.2047873
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发表时间：
1991-06-21
期刊：
影响因子：
56.9
通讯作者：
VENTER, JC
中科院分区：
文献类型：
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作者：
ADAMS, MD;KELLEY, JM;VENTER, JC
对随机选择的600多个人脑互补DNA（cDNA）克隆进行自动部分DNA测序，以产生表达序列标签（EST）。EST可用于发现新的人类基因、绘制人类基因组图谱和鉴定基因组序列中的编码区。在生成的序列中，337个代表新基因，其中48个与其他生物的基因具有显着相似性，例如酵母RNA聚合酶II亚基;果蝇驱动蛋白、Notch和分裂增强子;和鼠酪氨酸激酶受体。46个ESTs通过聚合酶链反应扩增后定位到染色体上。这种快速的cDNA表征方法将有助于在几年内以完全基因组测序的一小部分成本标记大多数人类基因，提供新的遗传标记，并作为不同生物研究领域的资源。
Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila kinesin, Notch, and Enhancer of split; and a murine tyrosine kinase receptor. Forty-six ESTs were mapped to chromosomes after amplification by the polymerase chain reaction. This fast approach to cDNA characterization will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing, provide new genetic markers, and serve as a resource in diverse biological research fields.