Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model
Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model
复制标题
用于尿道重建的上皮分化脂肪干细胞接种膀胱脱细胞基质移植物：动物模型
DOI：
10.1089/ten.tea.2013.0122
复制
发表时间：
2014-02-01
影响因子：
4.1
通讯作者：
Lv, Xiangguo
中科院分区：
文献类型：
--
作者：
Li, Hongbin;Xu, Yuemin;Lv, Xiangguo
可用上皮组织的数量有限被认为是尿道重建失败率高的主要原因。本研究的目的是探讨上皮分化兔脂肪源性干细胞（epithelial-differentiated rabbit fat -derived stem cells, epth - rascs）能否在体内功能上发挥上皮蛋白的作用，并成为尿路上皮的潜在替代品。我们使用5-溴-2 ' -脱氧尿苷（BrdU）标记后的epth - rascs植入膀胱脱细胞基质移植物（BAMGs）对雄性新西兰兔进行替代尿道成形术修复前尿道缺损，其基础是我们之前描述的体外上皮诱导系统。用无细胞bamg和未分化的rasc （un - rasc）种子bamg进行尿道成形术作为对照。术后经逆行尿道造影及肉眼观察，epth - rascs /BAMG组移植物挛缩明显改善，尿道连续性恢复。免疫荧光显示brdu标记的epth - rasc / un - rasc与细胞角蛋白13ormyosin共定位。与western blotting结果一致，在植入后早期，epth - rascs /BAMG组上皮细胞呈连续的局部多层结构，而BAMG组和un - rascs /BAMG组上皮细胞未见明显生长和局部单层生长。结果表明，上皮- rasc可作为尿路上皮的潜在替代品用于尿道组织工程，并可用于预防管腔挛缩及其并发症，包括复发性狭窄。
The limited amount of available epithelial tissue is considered a main cause of the high rate of urethral reconstruction failures. The aim of this study was to investigate whether epithelial-differentiated rabbit adipose-derived stem cells (Epith-rASCs) could play a role of epitheliumin vivofunctionally and be a potential substitute of urothelium. Substitution urethroplasty was performed to repair an anterior urethral defect in male New Zealand rabbits using Epith-rASCs seeded bladder acellular matrix grafts (BAMGs) after 5-bromo-2′-deoxyuridine (BrdU) labeling, based on thein vitroepithelial induction system we previously described. Urethroplasty with cell-free BAMGs and with undifferentiated rASCs (Und-rASCs) seeded BAMGs were performed as controls. After surgery, a notable amelioration of graft contracture and recovery of urethral continuity were observed in the Epith-rASCs/BAMG group by retrograde urethrograms and macroscopic inspection. Immunofluorescence revealed that the BrdU-labeled Epith-rASCs/Und-rASCs colocalized withcytokeratin 13ormyosin. Consistent with the results of western blotting, at early postimplantation stage, the continuous epithelial layer with local multilayered structure was observed in the Epith-rASCs/BAMG group, whereas no significant growth and local monolayer growth profile of epithelial cells were observed in the BAMG and Und-rASCs/BAMG group, respectively. The results showed that Epith-rASCs could serve as a potential substitute of urothelium for urethral tissue engineering and be available to prevent lumen contracture and subsequent complications including recurrent stricture.