Structure and Dynamics of Experimentally Introduced and Naturally Occurring Laccaria sp. Discrete Genotypes in a Douglas Fir Plantation
Structure and Dynamics of Experimentally Introduced and Naturally Occurring Laccaria sp. Discrete Genotypes in a Douglas Fir Plantation
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实验引入和天然存在的 Laccaria sp. 的结构和动力学。
DOI：
10.1128/aem.65.5.2006-2014.1999
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发表时间：
1999
影响因子：
4.4
通讯作者：
F. Le Tacon
中科院分区：
文献类型：
--
作者：
M. Selosse;F. Martin;D. Bouchard;F. Le Tacon
摘要 外生菌根真菌已被引入森林苗圃以促进幼苗生长。将接种过的幼苗移栽到人工林中引发了有关接种剂持久性及其对本土真菌种群影响的问题。我们之前表明（M.-A. Selosse et al. Mol. Ecol. 7:561–573, 1998），美国菌株 Laccaria bicolor S238N 在法国花旗松种植园外植后持续存在 10 年，没有基因渗入或自交，也没有在未接种的相邻地块上结果实。在本研究中，对种植园另一部分的这些结果与同域菌株的相关性进行了评估，该部分种植于 1985 年，幼苗接种了法国菌株 L. bicolor 81306 或未接种。约 720 Laccaria sp. 1994 年至 1997 年收集的子实体通过随机扩增的多态性 DNA 标记以及线粒体和核核糖体 DNA 的 PCR 扩增进行分型。所有地块都被小的自发离散基因型（基因）殖民。接种菌株 81306 在接种的树下结出丰富的果实，可能在本地蜡虫种群中基因渗入，但没有自交。与我们之前对 L. bicolor S238N 的调查相反，L. bicolor 81306 在一块未接种的树木上定殖。减数分裂分离分析证实入侵基因为菌株81306（P < 0.00058），意味着营养生长为1.1 m·year−1。 1998 年，用于接种种植园其他树木的 S238N 菌株也入侵了该地块。其他五个未接种的地块没有这些接种菌株。因此，接种菌株的命运与其说取决于其地理起源，不如说取决于未知的当地因素。
ABSTRACT Ectomycorrhizal fungi have been introduced in forest nurseries to improve seedling growth. Outplanting of inoculated seedlings to forest plantations raises the questions about inoculant persistence and its effects on indigenous fungal populations. We previously showed (M.-A. Selosse et al. Mol. Ecol. 7:561–573, 1998) that the American strainLaccaria bicolor S238N persisted 10 years after outplanting in a French Douglas fir plantation, without introgression or selfing and without fruiting on uninoculated adjacent plots. In the present study, the relevance of those results to sympatric strains was assessed for another part of the plantation, planted in 1985 with seedlings inoculated with the French strain L. bicolor 81306 or left uninoculated. About 720 Laccaria sp. sporophores, collected from 1994 to 1997, were typed by using randomly amplified polymorphic DNA markers and PCR amplification of the mitochondrial and nuclear ribosomal DNAs. All plots were colonized by small spontaneous discrete genotypes (genets). The inoculant strain 81306 abundantly fruited beneath inoculated trees, with possible introgression in indigenousLaccaria populations but without selfing. In contrast to our previous survey of L. bicolor S238N, L. bicolor 81306 colonized a plot of uninoculated trees. Meiotic segregation analysis verified that the invading genet was strain 81306 (P < 0.00058), implying a vegetative growth of 1.1 m · year−1. This plot was also invaded in 1998 by strain S238N used to inoculate other trees of the plantation. Five other uninoculated plots were free of these inoculant strains. The fate of inoculant strains thus depends less on their geographic origin than on unknown local factors.