Genomic changes in progression of low-grade gliomas
Genomic changes in progression of low-grade gliomas
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DOI：
10.1007/s11060-008-9644-z
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发表时间：
2008-11-01
影响因子：
3.9
通讯作者：
Delattre, Jean-Yves
中科院分区：
文献类型：
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作者：
Idbaih, Ahmed;Silva, Rosana Carvalho;Delattre, Jean-Yves
我们使用基于1兆碱基bac的阵列比较基因组杂交技术（aCGH），研究了一系列16例低级别胶质瘤（LGGs）及其随后向高级别恶性肿瘤的进展。原发肿瘤中最常见的染色体失衡是染色体7q、8q和22q的增加，以及染色体1p、13q和19q的丢失。在肿瘤进展中，染色体11q、7q、20q和21q的获得和染色体9p（包括CDKN2A位点、19q、14q、1p和6q）的丢失是最常见的基因组失衡。进展性肿瘤在染色体臂改变（平均异常染色体臂3.8比6.6）和BACs改变（17比21%）方面比原发肿瘤更不平衡。有趣的是，研究人员发现了与胶质瘤进展相关的新候选基因，特别是DOCK8、PTPRD、CER1、TPHO、DHFR、MSH3、ETS1、ACACA和CSE1L。
Using a one-megabase BAC-based array comparative genomic hybridization technique (aCGH), we have investigated a series of 16 low-grade gliomas (LGGs) and their subsequent progression to higher-grade malignancies. The most frequent chromosome imbalances in primary tumors were gains of chromosomes 7q, 8q, and 22q, and losses of chromosomes 1p, 13q, and 19q. In tumor progression, gains of chromosomes 11q, 7q, 20q, and 21q, and losses of chromosomes 9p, including CDKN2A locus, 19q, 14q, 1p, and 6q were the most frequent genomic disequilibria. Progressive tumors were more imbalanced than primary tumors in terms of altered chromosomal arms (3.8 vs. 6.6 in mean abnormal chromosomal arm) and altered BACs (17 vs. 21%). Interestingly, putative novel candidate genes associated with glioma progression were identified, in particular DOCK8, PTPRD, CER1, TPHO, DHFR, MSH3, ETS1, ACACA, and CSE1L.