Isolation and determination of major anthocyanin pigments in the pericarp of P. communis L. cv. 'Red Du Comices' and their association with antioxidant activity.
Isolation and determination of major anthocyanin pigments in the pericarp of P. communis L. cv. 'Red Du Comices' and their association with antioxidant activity.
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DOI：
10.5897/ajar11.1386
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Wu, J.
中科院分区：
文献类型：
--
作者：
Huang WenJiang, Huang WenJiang;Zhang ShaoLing, Zhang ShaoLing;Wu, J.
对梨果皮中的花色苷进行了研究。采用高效液相色谱/二极管阵列检测器（HPLC/DAD）和高效液相色谱/电喷雾离子化/质谱（HPLC/ESI/MS）分离、鉴定和定量“红杜康米斯”。通过将它们的质谱数据和保留时间与标准品和公开数据进行比较来鉴定单个花青素。花青素-3-半乳糖苷为主要成分，占总花色苷含量的93%。矢车菊素-3-半乳糖苷含量最高达23.73.2mg/100 g-1鲜重，其次为第八峰（2.14%）和矢车菊素-3-葡萄糖苷（1.23%）。首次从杜仲中鉴定出天竺葵素-3-芸香糖苷（pg-3-rutinoside）。2，2-二苯基-1-苦基肼（DPPH）和铁还原抗氧化力（FRAP）测定表明，红杜果皮提取物中含有花色苷，具有较强的抗氧化能力。
The anthocyanins in the fruit skin of Pyrus communis cv. 'Red Du Comice' were isolated, identified and quantified using high-performance liquid chromatography/diode array detection (HPLC/DAD) and HPLC/electrospray ionization/mass spectrometry (HPLC/ESI/MS). The individual anthocyanins were identified by comparing their mass spectral data and retention times with those of standards and published data. Cyanidin-3-galactoside was the major compound, taking up to 93% of the total anthocyanin content. The content of Cyanidin-3-galactoside reached 23.73.2 mg 100 g-1 fresh weight, followed by the eighth peak (2.14%) and Cyanidin-3-glucoside (1.23%). Pelargonidin-3-rutinoside (pg-3-rutinoside) was identified for the first time in P. communis L. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays showed that the extract from the fruit peel of 'Red Du Comice' contained anthocyanins and possessed high antioxidant capacity.