Two-dimensional crystals of the molecular chaperone GroEL reveal structural plasticity.
Two-dimensional crystals of the molecular chaperone GroEL reveal structural plasticity.
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分子伴侣 GroEL 的二维晶体揭示了结构可塑性。
DOI：
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发表时间：
1993
影响因子：
5.6
通讯作者：
W. Baumeister
中科院分区：
文献类型：
--
作者：
R. Zahn;J. Harris;G. Pfeifer;A. Plückthun;W. Baumeister
对于二维（2-D）结晶，我们已经纯化的分子伴侣GroEL从大肠杆菌的同质性。在纯化过程中结晶的最后和重要步骤是ATP-琼脂糖柱，在该柱上ATP和琼脂糖之间的间隔物连接到腺嘌呤的C8。采用云母铺展“负染-碳膜”法，以聚乙二醇为沉淀剂，得到了四种不同的二维周期阵列。其中有两颗是真正的晶石。一个晶体具有P2对称性，晶格常数为a = 24.3 nm和B = 16.9 nm，另一个晶体基本上具有P4对称性，并显示出晶格参数的平滑变化的局部变化（a = B = 23（+/- 1.3）nm）。在P4晶体中，非常引人注目的是每个GroEL粒子都偏离了GroEL典型的七重对称，这似乎是GroEL适应晶体对称所必需的。
For two-dimensional (2-D) crystallization we have purified the molecular chaperone GroEL from Escherichia coli to homogeneity. The final and important step for crystallization in the purification procedure was an ATP-agarose column, on which the spacer between ATP and agarose was attached to C8 of adenine. Using the mica spreading "negative staining-carbon film" procedure and polyethylene glycol as a precipitant, we obtained four different 2-D periodic arrays. Two of them turned out to be true crystals. One crystal has P2 symmetry and lattice constants of a = 24.3 nm and b = 16.9 nm, the other has essentially P4 symmetry and shows smoothly varying local changes in the lattice parameters (a = b = 23 (+/- 1.3) nm). Very striking in the P4 crystal is the departure within each individual GroEL particle from the GroEL-typical seven-fold symmetry, which seems to be required for GroEL to accommodate to a crystal symmetry.
DOI：
10.1016/s0021-9258(18)98800-9
发表时间：
1991-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. A. Mendoza;E. Rogers;G. Lorimer;Paul M. Horowitz
通讯作者：
J. A. Mendoza;E. Rogers;G. Lorimer;Paul M. Horowitz
大肠杆菌 groES 形态发生蛋白的纯化和性质。
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chandrasekhar,GN;Tilly,K;Woolford,C;Hendrix,R;Georgopoulos,C
通讯作者：
Georgopoulos,C