Cryopreparation and Electron Tomography of Yeast Cells.
Cryopreparation and Electron Tomography of Yeast Cells.
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酵母细胞的冷冻制备和电子断层扫描。
DOI：
10.1101/pdb.prot085589
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发表时间：
2017
影响因子：
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通讯作者：
Winey,Mark
中科院分区：
文献类型：
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作者：
O'Toole,EileenT;GiddingsJr,ThomasH;Winey,Mark
使用电子断层扫描技术对细胞进行三维成像，能够以前所未有的分辨率分析细胞结构。为了确保3D研究中细胞结构的最佳保存，使用快速冷冻和冷冻替代的方法准备用于断层扫描的细胞是必不可少的第一步。该方案概述了一种使用高压冷冻和冷冻替代获得保存完好的细胞的方法。我们发现，这种方法特别适合于电子断层扫描研究，并具有保留免疫电子显微镜抗原性的额外好处。还概述了对细胞成像和对细胞结构进行层析分析所涉及的步骤。
Three-dimensional imaging of cells using electron tomography enables analysis of cell structure at unprecedented resolution. The preparation of cells for tomography using rapid freezing followed by freeze-substitution is an essential first step to ensure the optimal preservation of the cell structure for 3D studies. This protocol outlines a method for obtaining well-preserved cells using high-pressure freezing followed by freeze-substitution. We have found that this method is particularly well suited for electron tomography studies and has the added bonus of preserving antigenicity for immuno-electron microscopy. The steps involved in imaging cells and performing tomographic analysis of cellular structures are also outlined.
酵母细胞的电子断层扫描。
影响因子：
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作者：
O'Toole,EileenT;Winey,Mark;McIntosh,JRichard;Mastronarde,DavidN
通讯作者：
Mastronarde,DavidN