An electrochemical immunosensor for simultaneous multiplexed detection of neuron-specific enolase and pro-gastrin-releasing peptide using liposomes as enhancer
An electrochemical immunosensor for simultaneous multiplexed detection of neuron-specific enolase and pro-gastrin-releasing peptide using liposomes as enhancer
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DOI：
10.1016/j.electacta.2011.04.012
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发表时间：
2011-06
影响因子：
6.6
通讯作者：
Z. Zhong;Na Peng;Y. Qing;Jin-lu Shan;Mengxia Li;W. Guan;N. Dai;Xianqing Gu;Dong Wang
中科院分区：
文献类型：
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作者：
Z. Zhong;Na Peng;Y. Qing;Jin-lu Shan;Mengxia Li;W. Guan;N. Dai;Xianqing Gu;Dong Wang
本研究以含有不同电化学活性分子的脂质体为信号增强剂，建立了同时定量神经元特异性烯醇化酶（NSE）和前胃泌素释放肽（ProGRP）的多重免疫分析方法。首先，分别制备了电化学活性分子抗坏血酸（AA）和尿酸（UA）的脂质体作为免疫标记物。采用夹心型免疫反应形式，将标记物包埋脂质体标记二抗，形成免疫复合物，加入表面活性剂破坏免疫复合物，用碳纳米管修饰电极电化学检测标记物。基于新的信号放大策略，免疫分析可同时检测浓度分别为50 - 1000 pg/mL和5.0 - 100 ng/mL的ProGRP和NSE。总之，与传统的平行单分析物免疫分析相比，多重免疫分析可以提供更高的样品通量、更少的样品消耗、更短的分析时间和更低的成本。
In this work, a multiplex immunoassay was constructed based on the amplification strategy using the liposomes which contained different electrochemical active molecule as singal enhancer for the simultaneous quantify neuron-specific enolase (NSE) and pro-gastrin-releasing peptide (ProGRP). First of all, the liposomes encapsulated with electrochemical active molecules, ascorbic acid (AA) and uric acid (UA), were prepared as immune labels respectively. With the sandwich type immunoreactions format, the marker entrapped liposomes labeled secondary antibodies were employed to form the immune complex, which were then destroyed by the addition of surfactant and the entrapped marker was electrochemically detected using carbon nanotubes modified electrodes. Based on the new signal amplification strategy, the immunoassay could simultaneous multiplexed detected ProGRP and NSE in the concentration range from 50 to 1000 pg/mL and 5.0 to 100 ng/mL, respectively. In conclusion, the multiplex immunoassay can offer higher sample throughput, less sample consumption, shorter assay time and lower cost than the traditional parallel single-analyte immunoassay.