Effect of viruses on luminol-dependent chemiluminescence of human neutrophils
Effect of viruses on luminol-dependent chemiluminescence of human neutrophils
复制标题
病毒对人中性粒细胞鲁米诺依赖性化学发光的影响
DOI：
10.1128/iai.24.3.673-678.1979
复制
发表时间：
1979
影响因子：
3.1
通讯作者：
P. Ogra
中科院分区：
文献类型：
--
作者：
H. Faden;P. Sutyla;P. Ogra
应用鲁米诺化学发光技术，研究了纽卡斯尔病、单纯疱疹病毒、牛痘、脑心肌炎、水泡性口炎和呼肠孤病毒对中性粒细胞功能的影响。纽卡斯尔病、单纯疱疹病毒和呼肠孤病毒分别抑制了98%、65%、46%和29%的化学发光，而脑心肌炎和水泡性口炎病毒则没有抑制作用。没有一种病毒影响吞噬作用或PMN活力。这些观察结果表明，在体外环境中与几种病毒的相互作用显着改变中性粒细胞的功能。这表明，在体内病毒感染过程中，PMN功能可能会发生类似的变化。
The effects of Newcastle disease, herpes simplex, vaccinia, encephalomyocarditis, vesicular stomatitis and reoviruses on in vitro function of neutrophils were studied in Ficoll-Hypaque-separated polymorphonuclear leukocytes (PMN) employing the technique of luminol-dependent chemiluminescence. Newcastle disease, herpes simplex vaccinia, and reoviruses depressed chemiluminescence by 98, 65, 46, and 29%, respectively, while encephalomyocarditis and vesicular stomatitis viruses had no inhibitory effect. None of the viruses affected phagocytosis or PMN viability. These observations suggest significant alteration of neutrophil function by interaction with several viruses in in vitro settings. It is suggested that similar changes in PMN function may occur during in vivo viral infection.