Pleurotus eryngii Polysaccharide Promotes Pluripotent Reprogramming via Facilitating Epigenetic Modification.
Pleurotus eryngii Polysaccharide Promotes Pluripotent Reprogramming via Facilitating Epigenetic Modification.
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DOI：
10.1021/acs.jafc.5b05661
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发表时间：
2016-02
影响因子：
6.1
通讯作者：
Wenwen Deng;Xia Cao;Yan Wang;Qingtong Yu;Zhijian Zhang;Rui Qu;Jingjing Chen;Genbao Shao;
中科院分区：
文献类型：
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作者：
Wenwen Deng;Xia Cao;Yan Wang;Qingtong Yu;Zhijian Zhang;Rui Qu;Jingjing Chen;Genbao Shao;
杏鲍菇是一种药用/食用菌，具有很高的营养价值和生物活性。它的多糖通过阳离子修饰已发展成为一种有效的基因载体。本研究用阳离子杏鲍菇多糖(CPS)与磷酸钙(CP)杂交，共传递载体(Oct4、Sox2、Klf4、c-Myc)，诱导分化为多能干细胞(IPSCs)。结果表明，与CP纳米颗粒相比，杂化纳米颗粒可以显著提高重编程的过程和效率(提高1.6倍)。在重新编程过程中，杂交CPS还促进了表观遗传修饰。此外，这些杂化纳米颗粒在其细胞内化过程中表现出多种途径(小窝和笼状蛋白介导的内吞作用)，这可以解释IPSCs生成的改善。因此，这些发现为杏鲍菇多糖通过主动表观遗传修饰在多潜能重编程中提供了一种新的应用。
Pleurotus eryngii is a medicinal/edible mushroom with great nutritional value and bioactivity. Its polysaccharide has recently been developed into an effective gene vector via cationic modification. In the present study, cationized P. eryngii polysaccharide (CPS), hybridized with calcium phosphate (CP), was used to codeliver plasmids (Oct4, Sox2, Klf4, c-Myc) for generating induced pluripotent stem cells (iPSCs). The results revealed that the hybrid nanoparticles could significantly enhance the process and efficiency of reprogramming (1.6-fold increase) compared with the CP nanoparticles. The hybrid CPS also facilitated epigenetic modification during the reprogramming. Moreover, these hybrid nanoparticles exhibited multiple pathways (both caveolae- and clathrin-mediated endocytosis) in their cellular internalization, which accounted for the improved iPSCs generation. These findings therefore present a novel application of P. eryngii polysaccharide in pluripotent reprogramming via active epigenetic modification.