Genetic heterogeneity found in the replicase gene of poinsettia mosaic virus isolates
Genetic heterogeneity found in the replicase gene of poinsettia mosaic virus isolates
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DOI：
10.1007/s00705-010-0708-y
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发表时间：
2010-05
影响因子：
2.7
通讯作者：
Yukari Okano;K. Maejima;Takuya Shiraishi;M. Hashimoto;H. Senshu;J. Ozeki;Shuichiro Takahashi;K. Komatsu;Y. Yamaji;S. Namba
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文献类型：
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作者：
Yukari Okano;K. Maejima;Takuya Shiraishi;M. Hashimoto;H. Senshu;J. Ozeki;Shuichiro Takahashi;K. Komatsu;Y. Yamaji;S. Namba
测定了5个日本一品红花叶病毒分离物（JN、JO 1、JO 2、JO 4和JO 5）的核苷酸全序列。这些分离株在基因组中含有一个大的开放阅读框架，与先前报道的两个欧洲分离株在核苷酸水平上的同源性为96.6-97.8%，在氨基酸水平上的同源性为91.3-98.1%。有趣的是，JO分离株被发现拥有8个共同的翻译移码位点之间的甲基转移酶和蛋白酶结构域的域间区域，导致相当大的变化相比，其他分离株的域间区域。这表明PnMV可能是通过这种翻译移码在其基因组中产生变异而进化的。
The complete nucleotide sequences of five isolates of poinsettia mosaic virus (PnMV) from Japan (JN, JO1, JO2, JO4, and JO5) were determined. These isolates contained a single large open reading frame in their genomes and shared 96.6–97.8% identity at the nucleotide level and 91.3–98.1% identity at the amino acid level with two previously reported European isolates. Interestingly, the JO isolates were found to possess eight common translational frameshift sites in the interdomain region between the methyltransferase and protease domains, resulting in considerable variation in the interdomain region compared to the other isolates. This suggests that PnMV might have evolved by creating variations in its genome by such translational frameshifts.