The release of granule enzymes from human neutrophils stimulated by aggregated immunoglobulins of different classes and subclasses.
The release of granule enzymes from human neutrophils stimulated by aggregated immunoglobulins of different classes and subclasses.
复制标题
不同类别和亚类聚集的免疫球蛋白刺激人中性粒细胞释放颗粒酶。
作者：
P. Henson;H. B. Johnson;H. Spiegelberg
已证明亚类IgG1、IgG2、IgG3、IgG4、IgA1和IgA2的聚集人骨髓瘤蛋白和正常IgG在无血清培养基中与人中性粒细胞反应，并诱导溶酶体酶β葡萄糖醛酸苷酶释放至细胞外部。相反，IgD，IgE和IgM巨球蛋白没有刺激这种释放。中性粒细胞与不溶性IgG和伊加聚集体的孵育导致聚集体粘附于中性粒细胞及其吞噬作用。在此过程中，颗粒酶的释放，但不是细胞质酶乳酸脱氢酶，发生，表明一个选择性的过程，而不是一个细胞裂解。可溶性聚集体没有诱导释放的酶与中性粒细胞在悬浮液中孵育时，但当绑定到过滤器作为nonphagocytosable表面，他们刺激贴壁细胞释放其颗粒内容物。后一过程先前已被证明是由于中性粒细胞颗粒直接挤出到外部。
Aggregated human myeloma proteins of subclasses IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2 and normal IgG have been shown to react with human neutrophils in a serum-free medium and to induce release of the lysosomal enzyme β glucuronidase to the exterior of the cells. In contrast, IgD, IgE and IgM macroglobulins did not stimulate this release. Incubation of neutrophils with insoluble IgG and IgA aggregates resulted in adherence of the aggregates to the neutrophils and their phagocytosis. During this process, release of granule enzymes, but not of the cytoplasmic enzyme lactic dehydrogenase, occurred, indicating a selective process rather than one of cell lysis. Soluble aggregates did not induce liberation of enzymes when incubated with the neutrophils in suspension, but when bound to micropore filters to serve as nonphagocytosable surfaces, they stimulated adherent cells to release their granule contents. This latter process has previously been shown to result from direct extrusion of neutrophil granules to the exterior.