Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.)
Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.)
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小麦 CDPK 基因家族的进化和功能研究 (Triticum aestivum L.)
DOI：
10.1007/s11103-007-9281-5
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发表时间：
2008-03-01
影响因子：
5.1
通讯作者：
Mao, Long
中科院分区：
文献类型：
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作者：
Li, Ai-Li;Zhu, Yuan-Fang;Mao, Long
钙依赖性蛋白激酶（CDPKs）是植物细胞在各种内源和环境刺激下钙离子浓度变化的重要传感器。本研究从面包小麦中鉴定了20个CDPK基因，并对其结构、功能和进化特征进行了全面的研究。使用各种方法获得了14个CDPKs的全长cDNA序列。小麦CDPKs在基因组结构、GC含量、亚细胞定位和亚群分类等方面与水稻CDPKs相似。对小麦CDPK基因对和小麦-水稻同源基因的分歧时间估计表明，大多数重复基因已经存在于小麦和水稻的共同祖先中。二倍体小麦基因组中CDPKs的数目估计至少为26个，接近于水稻、拟南芥和白杨中的数目。然而，EST序列之间的多态性发现所有三个同源等位基因的转录本为13出20 CDPKs。因此，六倍体小麦在其细胞中表达的CDPK基因应该比二倍体物种多2-3倍。小麦CDPK基因被发现对各种生物和非生物刺激有反应，包括寒冷、过氧化氢（H2 O2）、盐、干旱、白粉病（Blumeria graminis trillium，Bgt）以及植物激素脱落酸（阿坝）和赤霉酸（GA）。每个CDPK基因往往对多种处理有反应，表明小麦CDPK是多种信号转导途径的汇聚点。
Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-length cDNA sequences of 14 CDPKs were obtained using various approaches. Wheat CDPKs were found to be similar to their counterparts in rice in genomic structure, GC content, subcellular localization, and subgroup classification. Divergence time estimation of wheat CDPK gene pairs and wheat–rice orthologs suggested that most duplicated genes already existed in the common ancestor of wheat and rice. The number of CDPKs in diploid wheat genome was estimated to be at least 26, a number close to that in rice, Arabidopsis, and poplar. However, polymorphism among EST sequences uncovered transcripts of all three homoeologous alleles for 13 out of 20 CDPKs. Thus, the hexaploid wheat should have 2–3 fold more CDPK genes expressing in their cells than the diploid species. Wheat CDPK genes were found to respond to various biotic and abiotic stimuli, including cold, hydrogen peroxide (H2O2), salt, drought, powdery mildew (Blumeria graminis tritici, Bgt), as well as phytohormones abscisic acid (ABA) and gibberellic acid (GA). Each CDPK gene often responded to multiple treatments, suggesting that wheat CDPKs are converging points for multiple signal transduction pathways.