Bovine atrial natriuretic peptide in heart failure.
Bovine atrial natriuretic peptide in heart failure.
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牛心房钠尿肽治疗心力衰竭。
DOI：
10.1677/joe.0.1240463
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发表时间：
1990
影响因子：
4
通讯作者：
F. Marumo
中科院分区：
文献类型：
--
作者：
N. Takemura;H. Koyama;T. Sako;K. Ando;S. Motoyoshi;F. Marumo
本研究描述了轻度（细菌性心内膜炎; BEC）或重度（扩张型心肌病; DCM）心力衰竭的荷斯坦奶牛心房利钠肽（ANP）的浓度和分子形式。与对照牛（14.5 +/- 1.84 pmol/l，n = 12）相比，DCM（73.3 +/- 16.02 pmol/l，n = 4，P <0.01）和BEC（20.6 +/- 3.45 pmol/l，n = 7，P <0.05）牛的血浆ANP浓度显著增加。扩张型心肌病牛血浆心钠素水平显著高于BEC牛（P <0.01）。血浆ANP浓度与右房压（r = 0.95，P <0.01）和左室舒张末压（r = 0.84，P <0.01）呈显著正相关。血浆和右心房中ANP的凝胶渗透色谱显示一个单峰对应于血浆中真实人ANP（99-126）的洗脱位置，两个峰对应于心房提取物中真实人ANP（99-126）和pro-ANP。然而，在患有DCM的牛中，除了对应于ANP（99-126）的峰之外，还检测到对应于真实人β-ANP和/或前ANP的洗脱位置的峰。扩张型心肌病牛右心房心钠素含量显著高于对照牛和BEC牛（P <0.05）。因此，本研究提示，心房压力升高引起的心房扩张可能刺激心房心钠素的合成和分泌。（250字处删节）
The present study describes the concentration and molecular form of atrial natriuretic peptide (ANP) in Holstein dairy cattle with mild (bacterial endocarditis; BEC) or severe (dilated cardiomyopathy; DCM) heart failure. Significant increases in plasma concentration of ANP were observed in cattle with DCM (73.3 +/- 16.02 pmol/l, n = 4, P less than 0.01) and BEC (20.6 +/- 3.45 pmol/l, n = 7, P less than 0.05), when compared with those in control cattle (14.5 +/- 1.84 pmol/l, n = 12). The concentration of ANP in cattle with DCM was significantly (P less than 0.01) higher compared with that in cattle with BEC. Plasma concentration of ANP correlated significantly with right atrial pressure (r = 0.95, P less than 0.01) and left ventricular end-diastolic pressure (r = 0.84, P less than 0.01). Gel-permeation chromatography of ANP in plasma and the right atrium from control and cattle with BEC revealed a single peak corresponding to the elution position of authentic human ANP(99-126) in plasma, and two peaks corresponding to those of authentic human ANP(99-126) and pro-ANP in the atrial extract. In cattle with DCM, however, peaks corresponding to the elution positions of authentic human beta-ANP and/or pro-ANP were detected in addition to the peak corresponding to ANP(99-126). The content of ANP in the right atrium of cattle with DCM was significantly (P less than 0.05) increased compared with that in control cattle and those with BEC. The present study therefore suggests that the synthesis and secretion of ANP might be stimulated by atrial distention induced by increased atrial pressure.(ABSTRACT TRUNCATED AT 250 WORDS)