More sensitive high-pressure liquid-chromatographic determiantion of theophylline in serum.
More sensitive high-pressure liquid-chromatographic determiantion of theophylline in serum.
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更灵敏的高压液相色谱法测定血清中的茶碱。
DOI：
10.1093/clinchem/22.11.1903
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发表时间：
1976
影响因子：
9.3
通讯作者：
G. Schmidt
中科院分区：
文献类型：
--
作者：
R. F. Adams;F. L. Vandemark;G. Schmidt
我们提出了测定50毫升血清中茶碱的方法。将药物提取到含有内标8-氯茶碱的小体积溶剂中。提取液采用等容反相色谱法，在273 nm处测定洗脱的茶碱含量。在5- 20mg /l浓度范围内，每日重复性在5%以内。其他黄嘌呤和相关代谢物不受干扰。灵敏度为1mg /l。分光光度法测定结果的相关系数为0.989。阿莫巴比妥、二巴比妥、苯巴比妥和二苯基苯妥英不受干扰。单个样品的总分析时间为15分钟。
We present procedures for determining theophylline in 50 mul of serum. The drug is extracted into a small volume of solvent that contains an internal standard, 8-chlorotheophylline. The extract is Analyzed by isocratic reversedphase chromatography, with measurement of eluted theophylline at 273 nm. Day-to-day reproducibility within 5% is attainable for the concentration range 5--20 mg/liter. Other xanthines and related metabolites do not interfere. Sensitivity is 1 mg/liter. The correlation coefficient, when results by a spectrophotometric procedure were compared, was 0.989. Amobarbital, secobarbital, phenobarbital, and diphenylhydantoin do not interfere. Total analysis time for a single sample is 15 min.