DNA barcoding of crickets, katydids and grasshoppers (Orthoptera) from Central Europe with focus on Austria, Germany and Switzerland
DNA barcoding of crickets, katydids and grasshoppers (Orthoptera) from Central Europe with focus on Austria, Germany and Switzerland
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DOI：
10.1111/1755-0998.12638
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发表时间：
2017-09-01
影响因子：
7.7
通讯作者：
Haszprunar, G.
中科院分区：
文献类型：
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作者：
Hawlitschek, O.;Moriniere, J.;Haszprunar, G.
我们提出了一个DNA条形码的研究昆虫订单直翅目，是在三个国家，即条形码动物巴伐利亚州（德国），德国条形码的生活，奥地利条形码的生活和瑞士条形码的生活之间的合作项目。我们的数据集包括748 COI序列从127的162类群（78.4%）记录在三个国家。这122个物种中的93个（76.2%，包括所有Ensifera）可以使用DNA条形码可靠地识别。剩下的26个种类（蝗科和蚱科）形成了10个集群，在多达5个物种之间共享条形码，在3种情况下甚至跨越不同的属，在6种情况下甚至共享单个条形码。我们讨论不完整的谱系排序和杂交作为最可能的原因，这种现象，有关物种的遗传年轻和杂交以前已经观察到。我们还强调了核线粒体假基因（numts）的问题，一个已知的问题，在条形码的直翅目物种，和沃尔巴克氏体感染的可能性。最后，我们讨论了我们的条形码结果可能的分类学意义，并指出未来的研究方向。
We present a DNA barcoding study on the insect order Orthoptera that was generated in collaboration between four barcoding projects in three countries, viz. Barcoding Fauna Bavarica (Germany), German Barcode of Life, Austrian Barcode of Life and Swiss Barcode of Life. Our data set includes 748 COI sequences from 127 of the 162 taxa (78.4%) recorded in the three countries involved. Ninety-three of these 122 species (76.2%, including all Ensifera) can be reliably identified using DNA barcodes. The remaining 26 caeliferan species (families Acrididae and Tetrigidae) form ten clusters that share barcodes among up to five species, in three cases even across different genera, and in six cases even sharing individual barcodes. We discuss incomplete lineage sorting and hybridization as most likely causes of this phenomenon, as the species concerned are phylogenetically young and hybridization has been previously observed. We also highlight the problem of nuclear mitochondrial pseudogenes (numts), a known problem in the barcoding of orthopteran species, and the possibility of Wolbachia infections. Finally, we discuss the possible taxonomic implications of our barcoding results and point out future research directions.