Congo Red Stain Identifies Matrix Overproduction and Is an Indirect Measurement for c-di-GMP in Many Species of Bacteria
Congo Red Stain Identifies Matrix Overproduction and Is an Indirect Measurement for c-di-GMP in Many Species of Bacteria
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DOI：
10.1007/978-1-4939-7240-1_12
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Wozniak, Daniel J.
中科院分区：
文献类型：
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作者：
Jones, Christopher J.;Wozniak, Daniel J.
刚果红是一种重氮纺织染料，近一个世纪以来一直用于淀粉样纤维的可视化生产。微生物学方面的应用后来得到了发展，特别是在鉴定产生淀粉样附属物curli和在生物膜基质中过表达多糖的菌株方面。第二信使环二胍酸酯（c-di-GMP）调节生物膜基质多糖的产生，因此样品的刚果红染色可用于间接测量细菌中c-di-GMP产量的升高。刚果红允许以廉价、定量和高通量的方式鉴定产生高c-二gmp的菌株。
Congo red is a diazo textile dye that has been used to visualize the production of amyloid fibers for nearly a century. Microbiological applications were later developed, especially in identifying strains that produce amyloid appendages called curli and overexpressing polysaccharides in the biofilm matrix. The second messenger cyclic diguanylate (c-di-GMP) regulates the production of biofilm matrix polysaccharides, and therefore Congo red staining of samples can be utilized as an indirect measurement of elevated c-di-GMP production in bacteria. Congo red allows the identification of strains producing high c-di-GMP in an inexpensive, quantitative, and high-throughput manner.