Regulated ARE-mediated mRNA decay in Saccharomyces cerevisiae.
Regulated ARE-mediated mRNA decay in Saccharomyces cerevisiae.
复制标题
酿酒酵母中 ARE 介导的 mRNA 衰减的调节。
DOI：
10.1016/s1097-2765(01)00279-9
复制
发表时间：
2001
期刊：
影响因子：
16
通讯作者：
Peltz,SW
中科院分区：
文献类型：
--
作者：
Vasudevan,S;Peltz,SW
多种癌基因、细胞因子和生长因子转录物的稳定性通过存在于其3′-UTR中的ARE（富含AU的元件）受到信号通路的严格调节。我们已经鉴定了一种酵母转录物TIF 51 A，其稳定性通过其富含AU的3′-UTR调节。我们证明了哺乳动物TNFα和c-fosARE以类似的方式调节报告酵母转录物的周转。战神在葡萄糖培养基中稳定转录物，并在缺乏葡萄糖的培养基中或当Hog 1 p/p38 MAP激酶途径被抑制时作为不稳定元件发挥作用。值得注意的是，酵母和哺乳动物战神都促进酵母系统中的脱腺苷依赖性脱帽。此外，酵母ELAV同源物Pub 1 p调节TNFα ARE介导的稳定性。这些结果表明，酵母具有ARE介导的衰变的可调节机制，并建议从酵母到人类的这种周转过程的保护。
The stability of several oncogene, cytokine, and growth factor transcripts is tightly regulated by signaling pathways through an ARE (AU-rich element) present in their 3′-UTRs. We have identified a yeast transcript,TIF51A, whose stability is regulated through its AU-rich 3′-UTR. We demonstrate that the mammalian TNFα and c-fosAREs regulate turnover of a reporter yeast transcript in a similar manner. AREs stabilize the transcript in glucose media and function as destabilizing elements in media lacking glucose or when the Hog1p/p38 MAP kinase pathway is inhibited. Significantly, both yeast and mammalian AREs promote deadenylation-dependent decapping in the yeast system. Furthermore, the yeast ELAV homolog, Pub1p, regulates the stability mediated by the TNFα ARE. These results demonstrate that yeast possess a regulatable mechanism for ARE-mediated decay and suggest conservation of this turnover process from yeast to humans.