Green tea catechins and their metabolites in human skin before and after exposure to ultraviolet radiation.
Green tea catechins and their metabolites in human skin before and after exposure to ultraviolet radiation.
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DOI：
10.1016/j.jnutbio.2015.09.001
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发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
Williamson G
中科院分区：
文献类型：
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作者：
Clarke KA;Dew TP;Watson RE;Farrar MD;Osman JE;Nicolaou A;Rhodes LE;Williamson G
膳食黄酮类化合物可以防止皮肤晒伤炎症。使用不太完整的分析的初步报告表明，饮用绿茶后，在皮肤活检和水疱液中发现了某些儿茶素及其代谢物；然而，尚不清楚它们是否受到太阳模拟紫外线辐射（UVR）的影响，也不知道是否存在缀合形式，从而改变生物活性。本研究测试了以下假设：紫外线会影响健康志愿者饮用绿茶后皮肤中的儿茶素水平，以及血浆中的儿茶素与其在皮肤组织样本中的存在有何关系。在一项开放式口腔干预研究中，11 名受试者每天饮用绿茶和维生素 C 补充剂，持续 3 个月。收集补充前和补充后血浆样本、吸泡液和皮肤活检；后两个样品是在 UVR 之前和之后收集的。使用灵敏的高效液相色谱/质谱分析来测量完整的儿茶素代谢物、缀合物和游离形式。补充后，在皮肤活检中鉴定出 7 种绿茶儿茶素及其相应代谢物，其中 20 种在水疱液中，26 种在血浆中，其中 15 种绿茶儿茶素代谢物同时存在于水疱液和血浆中。戊内酯（O-甲基-M4-O-硫酸盐）是儿茶素的一种肠道微生物代谢物，在泡罩液样品中经 UVR 处理后，其含量显着增加 1.6 倍。综上所述，血浆和疱液中存在一些常见的儿茶素代谢物，且血浆中浓度较高。结果表明，绿茶儿茶素和代谢物在皮肤中具有生物利用度，并在皮肤和肠道微生物群中提取的儿茶素代谢物之间提供了新的联系。
Dietary flavonoids may protect against sunburn inflammation in skin. Preliminary reports using less complete analysis suggest that certain catechins and their metabolites are found in skin biopsies and blister fluid after consumption of green tea; however, it is not known if they are affected by solar-simulated ultraviolet radiation (UVR) or whether conjugated forms, with consequently altered bioactivity, are present. The present study tested the hypothesis that UVR affects the catechin levels in the skin of healthy volunteers after consumption of green tea and how catechins in the plasma are related to their presence in skin tissue samples. In an open oral intervention study, 11 subjects consumed green tea and vitamin C supplements daily for 3 months. Presupplementation and postsupplementation plasma samples, suction blister fluid and skin biopsies were collected; the latter two samples were collected both before and after UVR. A sensitive high-performance liquid chromatography/mass spectrometric assay was used to measure the intact catechin metabolites, conjugates and free forms. Seven green tea catechins and their corresponding metabolites were identified postsupplementation in skin biopsies, 20 in blister fluid and 26 in plasma, with 15 green tea catechin metabolites present in both blister fluid and plasma. The valerolactone, O-methyl-M4-O-sulfate, a gut microbiota metabolite of catechins, was significantly increased 1.6-fold by UVR in blister fluid samples. In conclusion, there were some common catechin metabolites in the plasma and blister fluid, and the concentration was always higher in plasma. The results suggest that green tea catechins and metabolites are bioavailable in skin and provide a novel link between catechin metabolites derived from the skin and gut microbiota.