Real-time single-nucleotide polymorphism profiling using Taqman technology for rapid recognition of Campylobacter jejuni clonal complexes
Real-time single-nucleotide polymorphism profiling using Taqman technology for rapid recognition of Campylobacter jejuni clonal complexes
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DOI：
10.1099/jmm.0.45971-0
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发表时间：
2005-10-01
影响因子：
3
通讯作者：
Bolton, FJ
中科院分区：
文献类型：
--
作者：
Best, EL;Fox, AJ;Bolton, FJ
空肠弯曲菌的快速鉴定对空肠弯曲菌的公共卫生调查具有重要意义。空肠感染多位点序列分型（MLST）提供的概念性进展已将克隆复合体确立为菌株水平上的重要流行病学组，从而能够准确且在遗传学上有效地鉴定C.空肠。基于MLST基因座等位基因的菌株相关单核苷酸多态性（SNP）等位基因歧视的实时PCR检测的发展为快速菌株检测提供了一种可能的方法。在对可用MLST数据进行详细分析后，确定了定义诊断最普遍的克隆复合体的关键等位基因的SNP。建立了检测ST-21、ST-45、ST-48、ST-61、ST-206和ST-257 6种主要克隆复合物特异性SNP的实时Taqman等位基因鉴别分析方法，可快速检测C.空肠分离株和初步菌株鉴定。这将为序列分型提供重要的补充技术，用于快速检测和菌株表征，以实时通知公共卫生管理和C.空肠感染
The rapid identification of Campylobacter jejuni isolates to strain level would significantly inform the public health investigation of C. jejuni infection. Conceptual advances provided by multilocus sequence typing (MLST) have established the clonal complex as an important epidemiological group at the strain level, enabling accurate and phylogenetically valid strain identification for C. jejuni. The development of real-time PCR assays for allelic discrimination of strain-associated single-nucleotide polymorphisms (SNPs) based upon MLST locus alleles offers one possible approach for rapid strain detection. SNPs defining key alleles diagnostic for the most prevalent clonal complexes were identified following a detailed analysis of the available MLST data. Real-time Taqman allelic discrimination assays designed to detect the SNPs specific for six major clonal complexes, ST-21, ST-45, ST-48, ST-61, ST-206 and ST-257, were developed, allowing the rapid detection of C. jejuni isolates and preliminary strain identification. This will provide an important complementary technique to sequence typing for rapid detection and strain characterization to inform in real-time the public health management and investigation of C. jejuni infections.