Effect of spermidine treatment on amino acid availability in amino acid-starved Escherichia coli.
Effect of spermidine treatment on amino acid availability in amino acid-starved Escherichia coli.
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DOI：
10.1016/0022-2836(68)90426-9
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发表时间：
1968-02
影响因子：
5.6
通讯作者：
David H. Ezekiel;Herma Brockman
中科院分区：
文献类型：
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作者：
David H. Ezekiel;Herma Brockman
在大肠杆菌中，亚精胺抑制蛋白质的合成，并刺激蛋白质的周转降解，从而使更多的氨基酸可用。测量高碘酸盐敏感性的tRNA从亚精胺处理的细胞，我们已经表明，亚精胺处理导致形成的氨基酸池的足够的大小，导致收费的tRNA。我们提出亚精胺在氨基酸饥饿细胞中刺激RNA合成（Raina & Cohen，1966）可以通过保留氨基酸来解释。
InEscherichia coli, spermidine inhibits the synthesis of protein and stimulates turnover degradation of protein, thus making more amino acids available. Measuring the periodate sensitivity of tRNA from spermidine-treated cells, we have shown that spermidine treatment leads to the formation of an amino acid pool of sufficient size to lead to charging of the tRNA. We propose that the stimulation of RNA synthesis by spermidine in amino acid-starved cells (Raina & Cohen, 1966) can be accounted for by the sparing of amino acids.