Whole vitreous humor dissection for vitreodynamic analysis.
Whole vitreous humor dissection for vitreodynamic analysis.
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DOI：
10.3791/52759
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发表时间：
2015-05-24
期刊：
影响因子：
--
通讯作者：
Humayun MS
中科院分区：
文献类型：
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作者：
Murali K;Kashani AH;Humayun MS
作者提出了一种有效的技术，以分离整个，完整的玻璃体核心和皮质从死后去核猪眼。虽然以前的研究已经显示了这种解剖的结果，但没有描述详细的步骤，这使得该领域以外的研究人员无法复制他们的方法。其他研究通过抽吸（其不保持玻璃体结构解剖学）或通过部分解剖（其仅分离玻璃体核心）来获取玻璃体。所提出的方法分离整个玻璃体，玻璃体核心和皮质完整，同时保持玻璃体解剖结构和结构的完整性。在该方法中，首先在摘除的猪眼中创建全厚度巩膜瓣，并且通过该巩膜瓣，脉络膜组织可以与巩膜分离。然后巩膜瓣扩张，脉络膜与巩膜完全分离。最后，将脉络膜视网膜组织从玻璃体剥离，留下分离的完整玻璃体。建议玻璃体解剖技术可用于研究玻璃体液的物理特性。特别是，这种方法对于涉及药物递送、玻璃体-视网膜氧转运和眼内对流的实验研究具有重要意义。
The authors propose an effective technique to isolate whole, intact vitreous core and cortex from post mortem enucleated porcine eyes. While previous studies have shown the results of such dissections, the detailed steps have not been described, precluding researchers outside the field from replicating their methods. Other studies harvest vitreous either through aspiration, which does not maintain the vitreous structure anatomy, or through partial dissection, which only isolates the vitreous core. The proposed method isolates the whole vitreous body, with the vitreous core and cortex intact, while maintaining vitreous anatomy and structural integrity. In this method, a full thickness scleral flap in an enucleated porcine eye is first created and through this, the choroid tissue can be separated from the sclera. The scleral flap is then expanded and the choroid is completely separated from the sclera. Finally the choroid-retina tissue is peeled off the vitreous to leave an isolated intact vitreous body. The proposed vitreous dissection technique can be used to study physical properties of the vitreous humor. In particular, this method has significance for experimental studies involving drug delivery, vitreo-retinal oxygen transport, and intraocular convection.