THE MEASUREMENT OF IONIC CONDUCTIVITIES AND MOBILITIES OF CERTAIN LESS COMMON ORGANIC IONS NEEDED FOR JUNCTION POTENTIAL CORRECTIONS IN ELECTROPHYSIOLOGY
THE MEASUREMENT OF IONIC CONDUCTIVITIES AND MOBILITIES OF CERTAIN LESS COMMON ORGANIC IONS NEEDED FOR JUNCTION POTENTIAL CORRECTIONS IN ELECTROPHYSIOLOGY
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DOI：
10.1016/0165-0270(94)00087-w
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发表时间：
1995-01-01
影响因子：
3
通讯作者：
BARRY, PH
中科院分区：
文献类型：
--
作者：
NG, B;BARRY, PH
在电生理学测量中，尤其是在使用膜片钳技术时，经常需要对连接电位进行校正。测量某些不太常见的有机离子的离子电导率和迁移率，用于电生理测量中的结电位校正。这些离子相对于K+的迁移率为：天冬氨酸（0.30）、胆碱（0.51）、葡萄糖酸（0.33）、谷氨酸（0.26）、HEPES（0.30）、羟乙基磺酸（0.52）、MES（0.37）、MOPS（0.35）、N-甲基-D-葡糖胺（0.33）和Tris（0.40）。此外，结电位直接测量了一些典型的简化膜片钳溶液中含有这些离子。测量的结电位通常与使用具有适当离子迁移率的广义亨德森方程计算的值一致。测量的准确性进行了讨论。
Junction potential corrections are often required in electrophysiological measurements, particularly when using the patch-clamp technique. The ionic conductivities and mobilities of certain less common organic ions were measured for use in junction potential corrections in electrophysiological measurements. The mobilities of these ions relative to K+ were: aspartate (0.30), choline (0.51), gluconate (0.33), glutamate (0.26), HEPES (0.30), isethionate (0.52), MES (0.37), MOPS (0.35), N-methyl-D-glucamine (0.33), and Tris (0.40). In addition, junction potentials were directly measured for a number of typical simplified patch-clamp solutions containing these ions. The measured junction potentials generally agreed well with the values calculated using the Generalized Henderson Equation with the appropriate ionic mobility. The accuracy of the measurements is discussed.