Endothelium dependence of dilation of pial arterioles in mouse brain by calcium ionophore.
Endothelium dependence of dilation of pial arterioles in mouse brain by calcium ionophore.
复制标题
钙离子载体对小鼠脑中软脑膜小动脉扩张的内皮依赖性。
DOI：
10.1161/01.str.19.11.1379
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发表时间：
1988
期刊：
影响因子：
8.3
通讯作者：
Nelson,GH
中科院分区：
文献类型：
--
作者：
Rosenblum,WI;Nelson,GH
先前的研究表明，软脑膜小动脉内皮的局部选择性原位损伤消除了乙酰胆碱或缓激肽产生的扩张。造成这种损伤的一种方法是在血管内伊文思蓝存在的情况下使用氦氖激光。由于乙酰胆碱或缓激肽产生的内皮依赖性扩张可能是通过与内皮表面受体的相互作用引发的，因此光可能只是使这些受体失活或破坏。我们使用钙离子载体 A-23187，这是另一种根据大动脉体外研究已知的内皮依赖性扩张剂，它将钙转移到内皮细胞中，而不是与表面受体相互作用。我们对 10 只小鼠的数据显示，在受伤之前，10(-5)MA-23187 将小动脉扩张至对照直径的 109 +/- 2%。氦氖激光选择性损伤内皮后，扩张基本上被消除（基线直径的 101 +/- 1%；p 小于 0.01，Wilcoxon 检验）。沿小动脉未受损的部位仍扩张至 A-23187。我们的数据表明，激光的作用不仅仅是使表面受体失活，而且是第一个体内微血管（直径小于 100 微米的血管）数据，显示对 A-23187 的反应具有内皮依赖性。
Previous studies have shown that local selective in situ injury of pial arteriolar endothelium eliminates the dilations produced by acetylcholine or bradykinin. One means of producing such injury employs a helium-neon laser in the presence of intravascular Evans blue. Since the endothelium-dependent dilations produced by acetylcholine or bradykinin may be initiated by interaction with endothelial surface receptors, it is possible that the light simply inactivates or destroys these receptors. We used calcium ionophore A-23187, another dilating agent known from in vitro studies of large arteries to be endothelium-dependent, which moves calcium into endothelial cells rather than interacting with surface receptors. Our data in 10 mice show that before injury, 10(-5)M A-23187 dilated arterioles to 109 +/- 2% of control diameter. After selective endothelial injury by helium-neon laser, dilation was essentially abolished (101 +/- 1% of baseline diameter; p less than 0.01, Wilcoxon test). Undamaged sites along the arteriole still dilated to A-23187. Our data indicate that the laser must do more than inactivate surface receptors and are the first in vivo microvascular (vessels of less than 100 micron diameter) data showing endothelium dependence of the response to A-23187.