Overexpression of Chloroplast Triosephosphate Isomerase Marginally Improves Photosynthesis at Elevated CO2 Levels in Rice
Overexpression of Chloroplast Triosephosphate Isomerase Marginally Improves Photosynthesis at Elevated CO2 Levels in Rice
复制标题
叶绿体磷酸三糖异构酶的过度表达在二氧化碳水平升高的情况下略微改善了水稻的光合作用
DOI：
10.1093/pcp/pcac115
复制
发表时间：
2022
影响因子：
4.9
通讯作者：
Makino A
中科院分区：
文献类型：
--
作者：
Suzuki Y;Shiina M;Takegahara-Tamakawa Y;Miyake C;Makino A
我们最近提出，叶绿体磷酸丙糖异构酶（cpTPI）通过磷酸丙糖利用能力（TPU）对水稻（Oryza sativa L.）在高CO2水平下的CO2同化速率（A）具有适度的控制作用。反义抑制研究的结果。在本研究中，在过表达编码cpTPI的基因的转基因水稻植株中研究了cpTPI过表达对光合作用的影响。两株转基因植株中cpTPI蛋白的表达量分别是野生型植株的4.8倍和12.1倍。增加的幅度大致对应于cpTPI转录水平的增加。在100和120 Pa的CO2水平下，转基因植物的Aat增加了6-9%，而在环境和低CO2水平下的Aat几乎没有受到影响。从A的CO2响应曲线估计的TPU容量观察到类似的增加。这些结果表明，cpTPI的过表达通过提高水稻中TPU的能力，在CO2水平升高时略微提高了光合作用。然而，在120 Pa的CO2水平下，转基因植物的生物量生产没有增加，这表明通过cpTPI过表达改善光合作用不足以改善水稻的生物量生产。
We recently suggested that chloroplast triosephosphate isomerase (cpTPI) has moderate control over the rate of CO2assimilation (A) at elevated CO2levels via the capacity for triose phosphate utilization (TPU) in rice (Oryza sativaL.) from its antisense-suppression study. In the present study, the effects of cpTPI overexpression on photosynthesis were examined in transgenic rice plants overexpressing the gene encoding cpTPI. The amounts of cpTPI protein in the two lines of transgenic plants were 4.8- and 12.1-folds higher than in wild-type plants, respectively. The magnitude of the increase approximately corresponded to the increase in transcript levels ofcpTPI. Aat CO2levels of 100 and 120 Pa increased by 6–9% in the transgenic plants, whereas those at ambient and low CO2levels were scarcely affected. Similar increases were observed for TPU capacity estimated from the CO2response curves ofA. These results indicate that the overexpression of cpTPI marginally improved photosynthesis at elevated CO2levels via improvement in TPU capacity in rice. However, biomass production at a CO2level of 120 Pa did not increase in transgenic plants, suggesting that the improvement in photosynthesis by cpTPI overexpression was not sufficient to improve biomass production in rice.