An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor
An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor
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DOI：
10.1016/j.burns.2005.10.015
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发表时间：
2006-05-01
期刊：
影响因子：
2.7
通讯作者：
Erdogan, D
中科院分区：
文献类型：
--
作者：
Alemdaroglu, C;Degim, Z;Erdogan, D
多项研究表明壳聚糖在促进伤口愈合方面是有效的。在本研究中，我们旨在开发一种含有表皮生长因子（EGF）的有效壳聚糖凝胶制剂，并确定其对大鼠二度烧伤伤口愈合的影响。制备了含2%壳聚糖凝胶且每毫升含10微克表皮生长因子的制剂。在一项体外研究中，为了探究表皮生长因子从制剂中的释放情况，24小时后释放率为97.3%。在体内研究中，将动物分为如下六组：磺胺嘧啶银[磺胺嘧啶银乳膏（SIL）]、含和不含表皮生长因子的壳聚糖凝胶组（EJ、J）、表皮生长因子溶液组（ES）以及未处理对照组[未烧伤（S）和未处理（Y）大鼠]应用组。在15秒暴露期间，用加热至94±1℃的水对背部皮肤造成均匀的深度二度烧伤。表皮生长因子制剂以0.160微克/平方厘米的剂量在烧伤区域重复应用14天（每天应用一次）。对组织样本进行免疫组织化学、组织化学和组织学评估伤口愈合情况。当对免疫组织化学结果进行评估时，观察到应用含表皮生长因子凝胶的组细胞增殖显著增加（p<0.05）。与未处理组相比，含表皮生长因子的壳聚糖凝胶组和表皮生长因子溶液组在伤口愈合方面显示出更好的效果（p<0.05）。组织学和组织化学评估结果也支持这些发现。这些结果表明，含表皮生长因子的壳聚糖凝胶可能是一种治疗二度烧伤的有前途的制剂。
Various studies have shown that chitosan is effective in promoting wound healing. In this study, we aimed to develop an effective chitosan gel formulation containing epidermal growth factor (EGF), and to determine the effect on healing of second-degree burn wounds in rats. Ten micrograms per millilitre EGF in 2% chitosan gel was prepared. In an in vitro study to investigate release of EGF from the formulations, the release rate was 97.3% after 24 h. In in vivo studies, animals were divided into six groups as follows: silver sulfadiazine [Silverdin(R) cream (SIL)], chitosan gel with and without EGF (EJ, J), EGF solution (ES) and untreated control groups [unburned (S) and untreated (Y) rats] applied groups, respectively. A uniform deep second-degree burn of the backskin was performed with water heated to 94 +/- 1 degrees C during a 15-s exposure. The EGF formulations were repeatedly applied on the burned areas with a dose of 0.160 mu g/cm(2) for 14 days (one application per day). Healing of the wounds was evaluated immunohistochemically, histochemically and histologically on the tissue samples. When the results were evaluated immunohistochemically, there were significant increases in cell proliferation observed in the EGF containing gel applied group (p