Skin wounds in the MRL/MPJ mouse heal with scar
Skin wounds in the MRL/MPJ mouse heal with scar
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DOI：
10.1111/j.1524-475x.2005.00092.x
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Lorenz, HP
中科院分区：
文献类型：
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作者：
Colwell, AS;Krummel, TM;Lorenz, HP
成年MRL/MPJ小鼠在耳部穿透伤修复过程中再生软骨。然而，这种小鼠品系通过再生或有疤痕愈合孤立皮肤伤口的能力尚不清楚。本研究的目的是研究MRL/MPJ小鼠皮肤创面的再上皮化率和胶原构筑，并与C57BL/6和Balb/c品系进行比较。在7周龄的MRL/MPJ、C57BL/6和Balb/c小鼠背部分别制作5 mm和2 mm的全厚切开和切除皮肤创面。同时在每只动物身上造成耳部穿孔伤。每种类型的皮肤伤口在48小时内完成再上皮化。所有切除创面在24、48和72小时均显示不完全再上皮化。14天时，所有皮肤创面均已基本愈合。与C57BL/6和Balb/c小鼠的耳部伤口相比，MRL/MPJ小鼠的耳部伤口在第28天完全愈合。第14天和第28天的背部皮肤创面切片显示，三种品系的小鼠之间有致密的胶原沉积和相似程度的纤维化。总而言之，与耳部伤口愈合相反，MRL/MPJ小鼠背部皮肤伤口愈合与C57BL/6和Balb/c小鼠相似，真皮胶原沉积和瘢痕形成。
Adult MRL/MpJ mice regenerate cartilage during repair of through-and-through ear punch wounds. However, the ability of this mouse strain to heal isolated cutaneous wounds by regeneration or with scar is unknown. The purpose of this study was to characterize the rate of reepithelialization and collagen architecture in dermal wounds from MRL/MpJ mice compared with C57bl/6 and Balb/c strains. Full-thickness incisional (5 mm) and excisional (2 mm diameter) skin wounds were made on the dorsum of 7-week-old MRL/MpJ, C57bl/6, and Balb/c mice. Ear punch wounds were made simultaneously on each animal. Reepithelialization was complete by 48 hours for incisional skin wounds in each strain. All excisional wounds showed incomplete reepithelialization at 24, 48, and 72 hours. At 14 days, all skin wounds had grossly healed. In contrast to the ear wounds made in C57bl/6 and Balb/c mice, MRL/MpJ ear wounds were completely healed by day 28. Dorsal skin wound sections at 14 and 28 days revealed dense collagen deposition and similar degrees of fibrosis between the three strains of mice. In conclusion, in contrast to wound healing in the ear, MRL/MpJ mouse dorsal cutaneous wounds heal similarly to C57bl/6 and Balb/c mice with dermal collagen deposition and scar formation.