Antiparasitic efficacy of dihydrosanguinarine and dihydrochelerythrine from Macleaya microcarpa against Ichthyophthirius multifiliis in richadsin (Squaliobarbus curriculus)
Antiparasitic efficacy of dihydrosanguinarine and dihydrochelerythrine from Macleaya microcarpa against Ichthyophthirius multifiliis in richadsin (Squaliobarbus curriculus)
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DOI：
10.1016/j.vetpar.2011.07.021
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发表时间：
2011-12-29
影响因子：
2.6
通讯作者：
Shen, Jin-yu
中科院分区：
文献类型：
--
作者：
Yao, Jia-yun;Zhou, Zhi-ming;Shen, Jin-yu
多filiisichthyophthirius multifiliis是一种侵入鱼类鳃和皮肤表面的全三毛原生动物，可引起世界上大多数淡水鱼的发病率和高死亡率。本文研究了小叶蝉叶粗提物和纯化合物的抗寄生虫活性。氯仿提取物对多叶蝉有良好的抗寄生活性。基于这些发现，氯仿提取物在生物活性引导下的硅胶柱层析分离得到两种显示有效活性的化合物。通过氢和碳-13核磁共振波谱和电子电离质谱鉴定了这两个化合物的结构，分别为二氢血碱和二氢chelerythrine。体内试验结果表明，二氢血碱和二氢赤藓碱对多家蝇具有较强的抗虫活性，EC50中值分别为5.18和9.43 mg/l。二氢血根碱和二氢chelerythrine对richadin的急性毒性（LC50）分别为13.3和18.2 mg/l。本研究结果为二氢血碱和二氢赤藓碱在治疗多毛线虫严重感染中的潜在应用提供了重要信息。(C) 2011 Elsevier B.V.版权所有
Ichthyophthirius multifiliis is a holotrichous protozoan that invades the gills and skin surfaces of fish and can cause morbidity and high mortality in most species of freshwater fish worldwide. The present study was undertaken to investigate the antiparasitic activity of crude extracts and pure compounds from the leaves of Macleaya microcarpa. The chloroform extract showed a promising antiparasitic activity against I. multifiliis. Based on these finding, the chloroform extract was fractionated on silica gel column chromatography in a bioactivity-guided isolation affording two compounds showing potent activity. The structures of the two compounds were elucidated as dihydrosanguinarine and dihydrochelerythrine by hydrogen and carbon-13 nuclear magnetic resonance spectrum and electron ionization mass spectrometry. The in vivo tests revealed that dihydrosanguinarine and dihydrochelerythrine were effective against I. multifiliis with median effective concentration (EC50) values of 5.18 and 9.43 mg/l, respectively. The acute toxicities (LC50) of dihydrosanguinarine and dihydrochelerythrine for richadsin were 13.3 and 18.2 mg/l, respectively. The overall results provided important information for the potential application of dihydrosanguinarine and dihydrochelerythrine in the therapy of serious infection caused by I. multifiliis. (C) 2011 Elsevier B.V. All rights reserved.