Glycolysis regulates the induction of lactate utilization for synaptic potentials after hypoxia in the granule cell of guinea pig hippocampus
Glycolysis regulates the induction of lactate utilization for synaptic potentials after hypoxia in the granule cell of guinea pig hippocampus
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DOI：
10.1016/j.neures.2004.08.008
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发表时间：
2004-12-01
影响因子：
2.9
通讯作者：
Yokono, K
中科院分区：
文献类型：
--
作者：
Takata, T;Yang, B;Yokono, K
乳酸被认为是一种替代底物，能够取代葡萄糖维持成年神经元的突触功能。但是，最近我们发现，在葡萄糖剥夺过程中，只有在NMDA和电压依赖性钙通道激活后，乳酸才能被用于维持突触电位。为了进一步阐明糖酵解和诱导乳酸利用之间的关系，我们测试了低氧条件下较低浓度的葡萄糖以诱导相对短缺的无氧能量产生。在10 mM葡萄糖培养基中缺氧后，乳酸盐不能维持群体峰值，但在暴露于较低浓度（5 mM）葡萄糖的缺氧后，乳酸盐可维持其原始水平。低血糖-缺氧期间的低温、NMDA通道阻滞剂和电压敏感性钙通道阻滞剂的浴用以及细胞外钙的缺失阻止了乳酸盐支持的群体尖峰的诱导。组织切片中的ATP水平在阻断乳酸盐支持的群体尖峰诱导的条件下相对保持。从这些观察，我们提出，在成年神经元中维持突触功能的能量来源从成年形式（葡萄糖单独）变化到未成熟的一个（葡萄糖和/或乳酸）后，缺乏葡萄糖供应。(C)2004年由Elsevier爱尔兰有限公司和日本神经科学学会出版。
Lactate is considered an alternative substrate that is capable of replacing glucose in maintaining synaptic function in adult neurons. But, we found recently that lactate could be utilized for maintenance of synaptic potentials only after the activation of NMDA and voltage-dependent-calcium channel during glucose deprivation. To clarify more on the relationship between glycolysis and induction of lactate utilization, we tested lower concentration of glucose with hypoxia to induce a relative shortage of anaerobic energy production. Population spikes are not maintained with lactate following hypoxia in 10 mM glucose medium, but are maintained at their original levels with lactate after exposure to hypoxia in lower concentration (5 mM) of glucose. Hypothermia during low glucose-hypoxia, bath application of the NMDA channel blocker and the voltage-sensitive calcium channel blocker, as well as the omission of extracellular calcium prevented the induction of the lactate-supported population spikes. ATP levels in the tissue slices are relatively preserved in the conditions that block the induction of lactate-supported population spikes. From these observations, we propose that the energy source for maintenance of synaptic function in adult neuron changes from adult form (glucose alone) to immature one (glucose and/or lactate) after short of glucose supply. (C) 2004 Published by Elsevier Ireland Ltd and the Japan Neuroscience Society.