Carcinoma in situ specimen classification based on intermediate cell measurements.
Carcinoma in situ specimen classification based on intermediate cell measurements.
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基于中间细胞测量的原位癌标本分类。
DOI：
10.1002/cyto.990080102
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
G. Vooijs
中科院分区：
文献类型：
--
作者：
M. Katzko;M. Pahlplatz;P. Oud;G. Vooijs
本研究探讨了通过测量外观正常的中间细胞来区分原位癌和正常癌的可能性。25例组织学证实的原位癌标本和99例正常标本，与年龄和使用或未使用口服激素避孕药的异常标本相匹配。涂片为单层涂片，用Thionin-Feulgen刚果红染色。测量了21个核特征。根据面积和平均光密度（AOD）这两个特征之间的关系，可以对实验组进行区分。对每个涂片的面积进行AOD回归。采用AOD水平与受试者年龄的相关性、变异系数、斜率、截距及平均值进行判别分析。这导致涂片分类的假阳性率为14%，漏报率为32%。当考虑到避孕措施的使用时，总体分类得到改善，假阳性率为12%，漏报率为20%。
In this study the possibility of classifying carcinoma in situ and normal specimens by measuring normal-appearing intermediate cells was explored. Twenty-five histologically verified carcinoma in situ specimens and 99 normal specimens, matched with the abnormal specimens for age and use or nonuse of an oral hormonal contraceptive, were examined. The smears were monolayer preparations stained with Thionin-Feulgen Congo red. Twenty-one nuclear features were measured. A discrimination among the experimental groups could be made on the basis of the relationship between two features, area and average optical density (AOD). A regression of AOD on area for each smear was performed. The correlation, coefficient of variation, slope, intercept, as well as the mean of the AOD level and the age of the subject were used in a discriminant analysis. This resulted in a smear classification with a false-positive rate of 14% and a missed-positive rate of 32%. When contraceptive use was taken into account the overall classification was improved with a false-positive rate of 12% and a missed-positive rate of 20%.