The effect of the degree of left renal vein constriction on the development of adolescent varicocele in Sprague-Dawley rats.
The effect of the degree of left renal vein constriction on the development of adolescent varicocele in Sprague-Dawley rats.
复制标题
左肾静脉收缩程度对Sprague-Dawley大鼠青少年静脉曲张的发展的影响。
DOI：
10.4103/1008-682x.157398
复制
发表时间：
2016-05
影响因子：
2.9
通讯作者：
Sun XZ
中科院分区：
文献类型：
--
作者：
Yao B;Zhou WL;Han DY;Ouyang B;Chen X;Chen SF;Deng CH;Sun XZ
实验模型允许调查精索静脉曲张（VC）的病理生理学超出了人类患者的可能。本研究采用大鼠随机对照实验，探讨左肾静脉缩窄程度对青少年VC发生的影响。将50只青春期雄性Sprague-Dawley大鼠（Rattus norvegicus）随机分为5组，每组10只：实验组（I-IV）分别用直径为0.5、0.6、0.7和0.8 mm的针头部分结扎左肾静脉。对照组（V）行假手术。分别于术前及术后30天测量左精索静脉内径。此外，用肉眼检查左肾的病变，并通过Masson三色染色进行评估。在I-IV组中分别有2只（20%）、4只（40%）、7只（70%）和10只（100%）大鼠成功诱导VC。其他大鼠主要由于左肾萎缩而未发生VC。Ⅲ组和Ⅳ组VC大鼠术后LSV直径分别为1.54 ± 0.16和1.49 ± 0.13 mm（P > 0.05），其增量分别为1.36 ± 0.10和1.31 ± 0.10 mm（P > 0.05）。这些结果表明，适当的左肾静脉缩窄是青少年VC发展的关键。此外，0.8 mm直径的针头可能更适合于在青春期大鼠模型中诱导左肾静脉缩窄。
Experimental models have allowed inquiry into the pathophysiology of varicocele (VC) beyond that possible with human patients. A randomized controlled study in rats was designed to clarify the influence of the degree of left renal vein constriction on the development of adolescent VC. Fifty adolescent male Sprague–Dawley rats (Rattus norvegicus) were randomly assigned to five groups of 10: the experimental groups (I–IV) underwent partial ligation of left renal veins with 0.5-, 0.6-, 0.7-, and 0.8-mm diameter needles, respectively. The control group (V) underwent a sham operation. The diameter of the left spermatic vein (LSV) was measured at baseline and 30 days postoperatively. In addition, the lesion of the left kidney was examined with the naked eye and assessed by Masson's trichrome staining. VC was successfully induced in 2 (20%), 4 (40%), 7 (70%), and 10 (100%) rats in groups I–IV, respectively. The other rats failed to develop VCs primarily due to left renal atrophy. No VC was observed in group V. The postsurgical LSV diameters in VC rats in groups III and IV were 1.54 ± 0.16 and 1.49 ± 0.13 mm, respectively (P > 0.05), and their increments were 1.36 ± 0.10 and 1.31 ± 0.10 mm, respectively (P > 0.05). These results suggest that suitable constriction of the left renal vein is critical for adolescent VC development. In addition, the 0.8-mm diameter needle may be more suitable for inducing left renal vein constriction in adolescent rat models.