Different populations of macrophages use either the vitronectin receptor or the phosphatidylserine receptor to recognize and remove apoptotic cells.
Different populations of macrophages use either the vitronectin receptor or the phosphatidylserine receptor to recognize and remove apoptotic cells.
复制标题
DOI：
10.4049/jimmunol.149.12.4029
复制
发表时间：
1992-12
影响因子：
4.4
通讯作者：
V. Fadok;J. Savill;C. Haslett;D. Bratton;D. Doherty;Priscilla A. Campbell;Peter M. Henson
中科院分区：
文献类型：
--
作者：
V. Fadok;J. Savill;C. Haslett;D. Bratton;D. Doherty;Priscilla A. Campbell;Peter M. Henson
许多组织中与程序性细胞死亡相关的关键特征之一是巨噬细胞对凋亡小体的吞噬作用。凋亡细胞的去除发生在裂解之前，表明这些细胞在凋亡发展过程中表达了巨噬细胞识别的特定表面变化。我们比较了四种不同巨噬细胞群识别凋亡细胞的机制。用两种不同的炎剂中的任一种诱导进入腹膜腔的小鼠巨噬细胞能够吞噬凋亡细胞。无论凋亡细胞的物种（人或鼠）或类型（淋巴细胞或中性粒细胞）如何，这种吞噬作用都会被磷脂酰丝氨酸（PS）抑制。相比之下，鼠骨髓巨噬细胞与人类单核细胞来源的巨噬细胞一样，利用玻连蛋白受体（一种αvβ3整合素）来去除凋亡细胞，无论其种类或类型如何。 PS脂质体抑制佛波酯处理的THP-1细胞的吞噬作用的观察表明，在某些情况下，人类巨噬细胞能够识别凋亡细胞上的PS。这些结果表明，巨噬细胞识别和吞噬凋亡细胞的机制是由所研究的巨噬细胞亚群决定的。
One of the key features associated with programmed cell death in many tissues is the phagocytosis of apoptotic bodies by macrophages. Removal of apoptotic cells occurs before their lysis, indicating that these cells, during the development of apoptosis, express specific surface changes recognized by macrophages. We have compared the mechanisms by which four different macrophage populations recognize apoptotic cells. Murine macrophages elicited into the peritoneal cavity with either of two different phlogistic agents were able to phagocytose apoptotic cells. This phagocytosis was inhibited by phosphatidylserine (PS), regardless of the species (human or murine) or type (lymphocyte or neutrophil) of the apoptotic cell. In contrast, the murine bone marrow macrophage, like the human monocyte-derived macrophage, utilized the vitronectin receptor, an alpha v beta 3 integrin, for the removal of apoptotic cells, regardless of their species or type. That human macrophages are capable, under some circumstances, of recognizing PS on apoptotic cells was suggested by the observation that PS liposomes inhibited phagocytosis by phorbol ester-treated THP-1 cells. These results suggest that the mechanism by which apoptotic cells are recognized and phagocytosed by macrophages is determined by the subpopulation of macrophages studied.