Msn2p and Msn4p control a large number of genes induced at the diauxic transition which are repressed by cyclic AMP in Saccharomyces cerevisiae
Msn2p and Msn4p control a large number of genes induced at the diauxic transition which are repressed by cyclic AMP in Saccharomyces cerevisiae
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DOI：
10.1128/jb.180.5.1044-1052.1998
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发表时间：
1998-03-01
影响因子：
3.2
通讯作者：
Jacquet, M
中科院分区：
文献类型：
--
作者：
Boy-Marcotte, E;Perrot, M;Jacquet, M
SNF1缺陷的多拷贝抑制因子Msn2p和Msn4p转录因子(MSn2/4p)通过逆境反应顺式元件(CCCCT)激活基因，以响应各种胁迫，该顺式元件也是环磷酸腺苷(CAMP)信号通路抑制的靶标。我们分析了MSN2msN4双突变株蛋白质合成的双向凝胶电泳谱，并与野生型相比，发现39个基因产物(包括ALD3、GDH3、GLK1、GPP2、Hsp104 HXK1、PGM2、SOD2、SSA3、SSA4、TKL2、TPS1和YBR149W)依赖MSN2/4p在双生转换中诱导表达，所有这些基因的表达都受到cAMP的抑制，在本研究中发现的其他30个基因在双生转换中仍然可以被诱导，这些基因中的一个亚集被发现在双生转换中被超诱导和其他受cAMP抑制的基因(包括ACH1、ADH2、ALD6、ATP2、GPD1、ICL1和KGD2)，我们根据这一分析得出结论，MSN2/4p控制着大量在二位体转换中诱导的基因，但另一种迄今尚未确定的调节因子也参与了这种反应。此外，我们在这里表明，cAMP抑制既适用于MSN2/4p依赖的和独立的二位位转换基因表达的控制，而且，所有MSN2/4p基因靶点都受到cAMP抑制的行为表明，这些调控因子可能是cAMP信号通路的靶标。
The multicopy suppressors of the snf1 defect, Msn2p and Msn4p transcription factors (Msn2/4p), activate genes through the stress-responsive cis element (CCCCT) in response to various stresses, This cis element is also the target for repression by the cyclic AMP (cAMP)-signaling pathway. We analyzed the two-dimensional gel electrophoresis pattern of protein synthesis of the msn2 msn4 double mutant and compared it with that of the wild-type strain during exponential growth phase and at the diauxic transition, Thirty-nine gene products (including those of ALD3, GDH3, GLK1, GPP2, HSP104 HXK1, PGM2, SOD2, SSA3, SSA4, TKL2, TPS1, and YBR149W) are dependent upon Msn2/4p for their induction at the diauxic transition, The expression of all these genes is repressed by cAMP, Thirty other genes identified during this study are still inducible in the mutant, A subset of these genes were found to be superinduced at the diauxic transition, and others were subject to cAMP repression (including ACH1, ADH2, ALD6, ATP2, GPD1, ICL1, and KGD2), We conclude from this analysis that Msn2/4p control a large number of genes induced at the diauxic transition but that other, as-yet-uncharacterized regulators, also contribute to this response, In addition, we show here that cAMP repression applies to both Msn2/4p-dependent and -independent control of gene Expression at the diauxic shift, Furthermore, the Tact that ail the Msn2/4p gene targets are subject to cAMP repression suggests that these regulators could be targets far the cAMP-signaling pathway.