The mucosal adhesion receptor α4β7 integrin is selectively increased in lymphocytes stimulated with lactoglobulin in children allergic to cow's milk
The mucosal adhesion receptor α4β7 integrin is selectively increased in lymphocytes stimulated with lactoglobulin in children allergic to cow's milk
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DOI：
10.1016/s0091-6749(99)70441-0
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发表时间：
1999-05-01
影响因子：
14.2
通讯作者：
Hauser, C
中科院分区：
文献类型：
--
作者：
Eigenmann, PA;Tropia, L;Hauser, C
背景资料：在小鼠中已经显示，整合素α 4 β 7指导记忆T细胞迁移到肠道相关淋巴组织中。然而，对食物过敏儿童T细胞归巢机制知之甚少。目的：我们研究了这种整合素和其他整合素在不同表现的牛奶过敏儿童中的表达（荨麻疹、特应性皮炎和喘息）。用β-乳球蛋白（牛奶中的主要变应原蛋白之一）和破伤风类毒素刺激PBMC，整合素的表达进行了研究，通过流式细胞仪分析后1周的culture.Results：我们发现显着较高的表达的α 4 β 7整合素的细胞从患者相比，对照组无过敏（P = .005）时，β-乳球蛋白被用来刺激细胞。α 4 β 7整联蛋白在β-乳球蛋白刺激的细胞中的表达水平也显著高于破伤风环状体刺激的细胞（P = 0.005）。α E β 7和α 4 β 1整联蛋白未被变应原刺激上调。大多数α 4 β 7整合素表达细胞被鉴定为CD 4（+）T细胞。结论：这些结果表明，β-乳球蛋白刺激后α 4 β 7整合素的表达与牛奶致敏的假定部位（即肠道相关淋巴组织，但与牛奶过敏症状部位无关）相关。
Background: It has been shown in mice that the integrin alpha 4 beta 7 directs the migration of memory T cells into the gut-associated lymphoid tissue. However, little is known about T-cell homing mechanisms in children with food allergies.Objective: We investigated the expression of this and other integrins in children with different manifestations of cow's milk allergy (urticaria, atopic dermatitis, and wheezing).Methods: PBMCs were stimulated with beta-lactoglobulin, 1 of the major allergenic proteins in cow's milk, and tetanus toxoid, Integrin expression was studied by flow cytometric analysis after 1 week of culture.Results: We found significantly higher expression of the alpha 4 beta 7 integrin in cells from patients compared with control subjects with no allergies (P = .005) when beta-lactoglobulin was used to stimulate the cells. alpha 4 beta 7 integrin was also expressed at significantly higher levels in beta-lactoglobulin-stimulated cells than in tetanus toroid-stimulated cells (P = .005). The alpha E beta 7 and the alpha 4 beta 1 integrins were not upregulated by allergen stimulation. Most alpha 4 beta 7 integrin-expressing cells were identified as CD4(+) T cells.Conclusion: These results show that alpha 4 beta 7 integrin expression after stimulation with beta-lactoglobulin correlates with the presumptive site of cow's milk sensitization (ie, the gut-associated lymphoid tissue but not with the site of symptoms of cow's milk allergy).