An enhancer complex confers both high-level and cell-specific expression of the human type X collagen gene
An enhancer complex confers both high-level and cell-specific expression of the human type X collagen gene
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DOI：
10.1016/s0014-5793(02)03606-2
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发表时间：
2002-11-20
期刊：
影响因子：
3.5
通讯作者：
Boot-Handford, RP
中科院分区：
文献类型：
--
作者：
Chambers, D;Young, DA;Boot-Handford, RP
X型胶原蛋白的表达仅限于软骨内生长板中的增生性软骨细胞。将含有人胶原X启动子的报告蛋白构建物瞬时转染到原代生长板软骨细胞中，鉴定出一种顺式作用的正调控DNA元件，该元件具有细胞特异性增强子特性，并与生长板软骨细胞中特异性表达的核蛋白结合。该区域的功能破坏导致报告基因转录激活的显著减少。所鉴定的增强子是控制X型胶原蛋白基因高水平和细胞特异性表达的主要元件。(C) 2002年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
Type X collagen expression is restricted to hypertrophic chondrocytes in the endochondral growth plate. Transient transfection of reporter constructs containing the human collagen X promoter into primary growth plate chondrocytes identified a cis-acting positive regulatory DNA element(s) that has cell-specific enhancer properties and binds a nuclear protein expressed specifically in growth plate chondrocytes. Functional disruption of this region results in a significant reduction in the activation of reporter gene transcription. The identified enhancer is a major element controlling both high-level and cell-specific expression of type X collagen gene. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.