Gonad histology and vitellogenin concentrations in brown trout (Salmo trutta) from Danish streams impacted by sewage effluent
Gonad histology and vitellogenin concentrations in brown trout (Salmo trutta) from Danish streams impacted by sewage effluent
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DOI：
10.1007/s10646-006-0061-9
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发表时间：
2006-04-01
期刊：
影响因子：
2.7
通讯作者：
Bjerregaard, Poul
中科院分区：
文献类型：
--
作者：
Bjerregaard, Lisette B.;Madsen, Allan H.;Bjerregaard, Poul
从一些受到污水影响的丹麦溪流中收集的褐鳟（Salmo trutta）被检查了性腺发育的改变和卵黄原蛋白合成的诱导。在2000/2001年6月/ 7月及2002年11月收集的鱼类中，有6条受污水影响的河流的雄鱼血浆卵黄原蛋白含量高于各自参考地点的雄鱼。建立了一种直接非竞争酶联免疫吸附测定褐鳟卵黄蛋白原的方法。在所有地点均观察到与污水暴露无明显关系的雌性双性人。在一组研究中，研究人员同时发现，卵黄原蛋白水平非常高的雄性褐鳟睾丸有高度的空泡化，而且只存在早期生精阶段，即精原细胞。这些睾丸结构改变的原因尚不清楚。然而，由于这些男性血浆卵黄原蛋白水平高表明雌激素暴露，空泡化也可能是内分泌干扰导致精子发生延迟或中断的结果。
Brown trout (Salmo trutta) collected from a number of Danish streams impacted by sewage effluent were examined for alterations to gonadal development and induction of vitellogenin synthesis. Among fish collected in June/July 2000/2001 and November 2002 higher levels of plasma vitellogenin were found in males from six streams impacted by sewage effluent compared to males from their respective reference sites. A direct non-competitive ELISA was developed for brown trout vitellogenin in order to perform the vitellogenin measurements. Intersex in females with no apparent relation to sewage effluent exposure was observed at all sites. In one stream, male brown trout with a very high level of vitellogenin were concomitantly found to have a high degree of vacuolation of the testes and a presence of only the early spermatogenic stage, spermatogonia. The cause of these alterations to the testis structure is unclear. However, as a high level of plasma vitellogenin in these males indicated estrogenic exposure, the vacuolation might also be a result of endocrine disruption causing delayed or disrupted spermatogenesis.