Search for progesterone receptors in testes from various animal species
Search for progesterone receptors in testes from various animal species
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寻找各种动物睾丸中的黄体酮受体
DOI：
10.1016/0303-7207(79)90005-4
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发表时间：
1979
影响因子：
4.1
通讯作者：
R. Tremblay
中科院分区：
文献类型：
--
作者：
J. Dubé;R. Tremblay
用[~3H]-R5020作为结合探针，我们在从海龟到人类的几种脊椎动物睾丸胞质中寻找孕酮受体的存在。此外，我们还在大鼠中测试了几种可能易诱导孕酮受体的实验条件。除了鸡，tfm患者和可能的青蛙，孕酮受体不能在任何其他动物物种中得到最终证明，也不能在大鼠中诱导。令人惊讶的是，他们不存在于斯坦利-Grumbeck假雌雄同体大鼠。在鸡睾丸中，孕酮受体的水平是可变的（3 - 45 fmol/mg蛋白）。在成人和高（87 fmol/mg蛋白）。在一天大的动物身上。孕激素受体是许多雌激素靶组织中雌二醇作用的标志物，其存在似乎与鸡睾丸在雌激素影响下的性逆转能力平行。在人类tfm中，这些受体的存在也可能表明性腺对雌激素敏感，或者在胚胎期是如此，尽管雌激素作用的性质还不清楚。
Using [3H]-R5020 as binding probe, we have looked for the presence of progesterone receptors in testis cytosol from a few vertebrate species ranging from the turtles to the humans. In addition, we have tested in the rat several experimental conditions potentially susceptible to induce progesterone receptors. With the exception of chicken, tfm patients and possibly the frogs, progesterone receptors could not be conclusively demonstrated in any of the other animal species tested nor could they be induced in the rat. Surprisingly, they were not present in Stanley-Grumbeck pseudohermaphrodite rats. In chicken testes, the levels of progesterone receptor were variable (3–45 fmol per mg prot.) in the adults and high (87 fmol per mg prot.) in one-day-old animals. The presence of progesterone receptor, a marker of estradiol action in many estrogen-target tissues, seems to be parallel to the capacity of chicken testes for sex reversal under the influence of estrogens. In human tfm, the presence of these receptors could also indicate that the gonads are estrogen-sensitive or that they were so during embryonic life although the nature of estrogenic action is not known.