Recognition of sugars on surface-bound cap-shaped gold particles modified with a polymer brush
Recognition of sugars on surface-bound cap-shaped gold particles modified with a polymer brush
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DOI：
10.1016/j.colsurfb.2007.01.003
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发表时间：
2007-05-15
影响因子：
5.8
通讯作者：
Hakari, Mami
中科院分区：
文献类型：
--
作者：
Anraku, Yasutaka;Takahashi, Yoshiko;Hakari, Mami
通过光隐法制备的苯硼酸残基二硫代无规共聚物（Cys-Poly（3-丙烯酰胺苯基硼酸-co-2-二甲氨基丙基甲基丙烯酰胺），Cys-Poly(APBA-co-DMAPMA)）作为聚合物刷在真空蒸发金膜上沉积的帽状金颗粒上，并将该聚合物刷作为糖蛋白、卵清蛋白（OVA）、用紫外-可见光谱和表面等离子体共振对其进行了检测。采用原子转移自由基聚合（ATRP）法制备了二硫代甘露糖载体二硫代聚（2-甲基丙烯酰氧乙基- d-甘露pyranoside） （DT-PMEMan），构建了类似的体系。用该聚合物组成的毛刷作为凝集素刀豆蛋白a （cona）的传感元件进行了检测。用Cys-Poly（APBA-co-DMAPMA）和DT-PMEMan修饰的传感器细胞分别显示出OVA和Con a的浓度依赖性结合，其检测限与在玻璃基板上沉积单层聚合物刷涂金颗粒的检测限相当。使用这个系统。可以期待为固定在固体基材上的帽状金颗粒上的各种功能聚合物刷开辟一个新的视角。(c) 2007 Elsevier B.V.版权所有
A dithiolated random copolymer with pendent phenylboronic acid residues (Cys-Poly(3-acrylamidophenylboronic acid-co-2-dimethylaminopropyl methacrylamide), Cys- Poly (APBA-co-DMAPMA)) obtained by photo-iniferter method was accumulated as a polymer brush on a capshaped gold particles deposited on a vacuum-evaporated gold film, and the usefulness of the polymer brush as a sensing element for glycoprotein, ovalbumin (OVA), was examined by using UV-vis spectroscopy with a help of surface plasmon resonance. A similar system was constructed with a dithiolated mannose-carrying polymer, dithiolated-poly(2-methacryloyloxyethyl-D-mannopyranoside) (DT-PMEMan), prepared by the atom transfer radical polymerization (ATRP). The brush composed of this polymer was examined as a sensing element for lectin, concanavalin A (Con A). The sensor cells modified with Cys-Poly(APBA-co-DMAPMA) and DT-PMEMan showed a concentration-dependent binding of OVA and Con A, respectively, with a comparable detection limit to those with a monolayer of polymer brush-coated gold particle deposited on a glass substrate. Using this system. it can be expected to open a new perspective to various functional polymer brushes fixed to the cap-shaped gold particle on a solid substrate. (c) 2007 Elsevier B.V. All rights reserved.