The localization of matrix metalloproteinase-20 (MMP-20, enamelysin) in mature human teeth
The localization of matrix metalloproteinase-20 (MMP-20, enamelysin) in mature human teeth
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DOI：
10.1177/154405910208100905
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发表时间：
2002-09-01
影响因子：
7.6
通讯作者：
Tjäderhane, L
中科院分区：
文献类型：
--
作者：
Sulkala, M;Larmas, M;Tjäderhane, L
在牙本质形成过程中，成牙本质细胞中也检测到基质金属蛋白酶家族成员--基质金属蛋白酶-20(釉质溶解蛋白)。我们研究了基质金属蛋白酶-20在健康和疾病成人牙齿中的存在和定位。在免疫组织化学中，基质金属蛋白酶-20在龋损的牙根成牙本质细胞层和扩张的牙本质小管中表达最强。免疫印迹法检测到正常和龋齿的成牙本质细胞和牙髓组织、健牙的牙本质液和牙本质中均有MMP20表达，而软龋牙本质中未见表达。我们的结论是，在牙本质形成过程中产生的基质金属蛋白酶-20被结合到牙本质中，并可能在龋病进展过程中释放出来。牙本质-牙髓复合体中基质金属蛋白酶-20的主要细胞来源是成牙本质细胞，它将基质金属蛋白酶-20分泌到牙本质液中。
MMP-20 (enamelysin), the matrix metalloproteinase family member discovered in the enamel organ, has also been detected in odontoblasts during dentin formation. We studied the presence and localization of MMP-20 in mature human teeth in health and disease. In immunohistochemistry, MMP-20-positive staining was observed most intensively in the radicular odontoblastic layer and also in dilated dentinal tubuli of caries lesions. By Western blotting, MMP-20 was detected in odontoblasts and pulp tissue of both sound and carious teeth, in dentinal fluid and dentin of sound teeth, but not in soft carious dentin. We conclude that MMP-20 produced during primary dentinogenesis is incorporated into dentin and may be released during caries progression. The main cellular source of MMP-20 in the dentin-pulp complex is the odontoblasts, which secrete MMP-20 into the dentinal fluid.