Transcriptional Regulation and Spatial Organisation of the Human AML1/RUNX1 Gene
Transcriptional Regulation and Spatial Organisation of the Human AML1/RUNX1 Gene
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DOI：
10.1002/jcb.23117
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发表时间：
2011-08-01
影响因子：
4
通讯作者：
Razin, Sergey V.
中科院分区：
文献类型：
--
作者：
Markova, Elena N.;Kantidze, Omar L.;Razin, Sergey V.
转录因子RUNX 1是脊椎动物造血的关键调节因子。在人类中，编码这种转录因子的260 kb长的基因位于21号染色体上。该基因由两个可选的启动子转录，这两个可选的启动子通常被称为远端启动子和近端启动子。在模型实验中，发现这两种启动子在不同谱系的细胞中具有活性，尽管RUNX 1优先在造血细胞中表达。在本研究中，我们试图确定的调控元件，可以指导组织特异性表达的RUNX 1基因。在RUNX 1基因中发现了两个这样的调控元件。这些元件之一，位于内含子1内，是造血特异性增强子。位于内含子5.2内的第二个调控元件有助于形成活性染色质枢纽，其整合上述增强子和P1和P2启动子。J.细胞。112：1997-2005，2011. (C)2011 Wiley-Liss，Inc.
The transcription factor RUNX1 is a key regulator of haematopoiesis in vertebrates. In humans, the 260-kb long gene coding for this transcription factor is located on chromosome 21. This gene is transcribed from two alternative promoters that are commonly referred to as the distal and the proximal promoters. In model experiments, these two promoters were found to be active in cells of different lineages, although RUNX1 is preferentially expressed in haematopoietic cells. In the present study, we attempted to identify the regulatory elements that could guide tissue-specific expression of the RUNX1 gene. Two such regulatory elements were found within the RUNX1 gene. One of these elements, located within intron 1, is a haematopoietic-specific enhancer. The second regulatory element, located within intron 5.2, contributes to the formation of an active chromatin hub, which integrates the above-mentioned enhancer and the P1 and P2 promoters. J. Cell. Biochem. 112: 1997-2005, 2011. (C) 2011 Wiley-Liss, Inc.