Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis
Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis
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DOI：
10.1128/jb.00968-07
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发表时间：
2007-10-01
影响因子：
3.2
通讯作者：
Kudo, Toshiaki
中科院分区：
文献类型：
--
作者：
Kajiyama, Yusuke;Otagiri, Masato;Kudo, Toshiaki
枯草芽孢杆菌MRP(又称SHA)是由mrpABCDEFG编码的一种特殊的Na+/H+逆向转运蛋白。利用HIS标记的MRP蛋白，我们通过下拉和蓝色天然聚丙烯酰胺凝胶电泳法显示mrpABCDEFG基因产物形成复合体。这是第一个证明MRP是阳离子-质子逆向转运蛋白3家族中的多组分逆向转运蛋白的分子证据。
The Bacillus subtilis Mrp (also referred to as Sha) is a particularly unusual Na+/H+ antiporter encoded by mrpABCDEFG. Using His tagging of Mrp proteins, we showed complex formation by the mrpABCDEFG gene products by pull-down and blue native polyacrylamide gel electrophoresis analyses. This is the first molecular evidence that the Mrp is a multicomponent antiporter in the cation-proton antiporter 3 family.