Rapid epidemiologic assessment of glucose-6-phosphate dehydrogenase deficiency in malaria-endemic areas in Southeast Asia using a novel diagnostic kit
Rapid epidemiologic assessment of glucose-6-phosphate dehydrogenase deficiency in malaria-endemic areas in Southeast Asia using a novel diagnostic kit
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DOI：
10.1111/j.1365-3156.2004.01237.x
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发表时间：
2004-05-01
影响因子：
3.3
通讯作者：
Kawamoto, F
中科院分区：
文献类型：
--
作者：
Jalloh, A;Tantular, IS;Kawamoto, F
我们最近报道了一种新的快速筛查葡萄糖-6-磷酸脱氢酶（G6 PD）缺乏症的方法。该方法采用了一种新的甲瓒底物（WST-8），能够定性和定量检测杂合子女性。在这里，我们报告在缅甸和印度尼西亚的三个疟疾中心和疟疾流行的村庄进行实地调查期间，单独或与现场快速诊断疟疾相结合的评估。在缅甸的855名受试者中共发现57例重度（45名男性和12名女性）和34例轻度（5名男性和29名女性）G6 PD缺乏症病例，而在印度尼西亚的1286名受试者中发现30例重度（25名男性和5名女性）和23例轻度（6名男性和17名女性）病例。在所有病例中，严重缺乏症均用另一种甲瓒法确认，但由于其检测阈值的限制，后者将轻度病例误诊为G6 PD正常。我们的研究结果表明，新的方法可以定性检测严重缺陷的主题，以及杂合子女性在该领域。在缅甸，抗疟疾药物伯氨喹被安全地用于间日疟原虫感染患者。我们新的快速筛查方法可能对诊断G6 PD缺乏症至关重要，特别是在没有电力的农村地区，并可推荐用于疟疾控制计划。
We recently reported a new rapid screening method for glucose-6-phosphate dehydrogenase (G6PD) deficiency. This method incorporates a new formazan substrate (WST-8) and is capable of detecting heterozygous females both qualitatively and quantitatively. Here, we report its evaluation during field surveys at three malaria centres and in malaria-endemic villages of Myanmar and Indonesia, either alone or in combination with a rapid on-site diagnosis of malaria. A total of 57 severe (45 males and 12 females) and 34 mild (five males and 29 females) cases of G6PD deficiency were detected among 855 subjects in Myanmar whilst 30 severe (25 males and five females) and 23 mild (six males and 17 females) cases were found among 1286 subjects in Indonesia. In all cases, severe deficiency was confirmed with another formazan method but due to limitations in its detection threshold, mild cases were misdiagnosed as G6PD-normal by this latter method. Our results indicate that the novel method can qualitatively detect both severely deficient subjects as well as heterozygous females in the field. The antimalarial drug, primaquine, was safely prescribed to Plasmodium vivax-infected patients in Myanmar. Our new, rapid screening method may be essential for the diagnosis of G6PD deficiency particularly in rural areas without electricity, and can be recommended for use in malaria control programmes.