A Miniaturized Blotting System for Simultaneous Detection of Different Autoantibodies

A Miniaturized Blotting System for Simultaneous Detection of Different Autoantibodies
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DOI:
10.1007/978-1-59745-542-8_15
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发表时间:
2009-01-01
期刊:
PROTEIN BLOTTING AND DETECTION: METHODS AND PROTOCOLS
影响因子:
--
通讯作者:
Bachmann, Michael
Bachmann, Michael
中科院分区:
其他
文献类型:
--
作者:
Canzler, Ulrich;Bartsch, Holger;Bachmann, Michael

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肿瘤患者的血清中经常含有针对肿瘤相关抗原的自身抗体。在这里,我们描述了一个小型化的免疫印迹平台,使我们能够筛选患者血清中存在的自身抗体,以10个自身抗原平行。
Sera of tumor patients frequently contain autoantibodies to tumor-associated antigens. Here we describe a miniaturized immunoblot platform allowing us to screen sera of patients for the presence of autoantibodies to ten autoantigens in parallel.